Document Detail

Selective preconcentration of amino acids and peptides using single drop microextraction in-line coupled with capillary electrophoresis.
MedLine Citation:
PMID:  20381809     Owner:  NLM     Status:  MEDLINE    
Single drop microextraction (SDME) can be in-line coupled with capillary electrophoresis by attaching a drop to the tip of a capillary. With a 2-layer drop comprised of an aqueous basic acceptor phase covered with a thin organic layer, acidic analytes in an aqueous acidic donor phase can be extracted into the organic layer and then back-extracted into the acceptor phase. However, preconcentration of amino acids and peptides by SDME is difficult since their zwitterionic properties prevent them from being partitioned in the middle organic phase. When amino acids were derivatized with 4-fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F), amino acids without a charged side chain were converted to carboxylic acids. In the acidic donor phase, those NBD-amino acids were predominantly neutral and they were successfully concentrated into the basic acceptor phase. In the meantime, amino acids with a charged side chain after NBD-F derivatization were not concentrated via SDME. With this selective SDME, we were able to extract acidic and neutral amino acids obtaining several hundred-fold enrichments within 5 min at 25 degrees C, while leaving basic amino acids-Arg, Lys, and His-in the acidic donor phase. Furthermore, detection sensitivity was enhanced by employing laser-induced fluorescence detection. We then applied this technique to the selective concentration of peptides.
Yang-Ki Park; Kihwan Choi; Ahmed Yacine Badjah Hadj Ahmed; Zeid A AlOthman; Doo Soo Chung
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-03-25
Journal Detail:
Title:  Journal of chromatography. A     Volume:  1217     ISSN:  1873-3778     ISO Abbreviation:  J Chromatogr A     Publication Date:  2010 May 
Date Detail:
Created Date:  2010-04-26     Completed Date:  2010-07-19     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9318488     Medline TA:  J Chromatogr A     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  3357-61     Citation Subset:  IM    
Copyright Information:
2010 Elsevier B.V. All rights reserved.
Department of Chemistry, Seoul National University, Seoul 151-747, South Korea.
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MeSH Terms
4-Chloro-7-nitrobenzofurazan / analogs & derivatives,  chemistry
Amino Acids / chemistry*
Chemical Fractionation / methods*
Electrophoresis, Capillary / methods*
Molecular Structure
Peptides / chemistry*
Reg. No./Substance:
0/Amino Acids; 0/Peptides; 10199-89-0/4-Chloro-7-nitrobenzofurazan; 29270-56-2/7-fluoro-4-nitrobenzo-2-oxa-1,3-diazole

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