Document Detail


Selective polyunsaturated fatty acids enrichment in phospholipids from neuronal-derived cell lines.
MedLine Citation:
PMID:  16337275     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Most studies aimed at exploring the molecular and cellular properties of plasma membranes in neural tissues make use of cell lines. However, cell membrane lipid composition of cell lines is notably different from that of brain tissues where they presumably derive from. Using septal-derived SN56 cells and hippocampal-derived HT22 cells, we demonstrated that cell lines exhibit lower contents of saturated (18:0) and long polyunsaturated fatty acids (PUFA; 20:4n-6 and especially 22:6n-3), as well as higher monounsaturated fatty acid contents (mainly 18:1n-9), compared to mouse brain. Also, cell lines exhibited higher contents of sterol esters and lower contents of cholesterol and phospholipids, especially phosphatidylethanolamine and phosphatidylserine. We have also evaluated the effects of different (n-3/n-6) PUFA enrichments on fatty acid and phospholipid contents in these cell lines. Our results show that enrichment of culture medium with 22:6n-3 and 20:4n-6 in a 70/30 proportion during 48 h, using fat-free bovine serum albumin as vehicle, successfully readjusted fatty acid profiles in cell line-polar lipids to values found in natural nerve cells. Interestingly, no differences in cell survival were observed upon enrichment. The generalization of these methodologies would allow a more feasible adaptation of cellular models to the study of in vivo nerve physiology.
Authors:
Virginia Martín; Eduardo Almansa; Noemí Fabelo; Mario Díaz
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't     Date:  2005-12-06
Journal Detail:
Title:  Journal of neuroscience methods     Volume:  153     ISSN:  0165-0270     ISO Abbreviation:  J. Neurosci. Methods     Publication Date:  2006 Jun 
Date Detail:
Created Date:  2006-05-15     Completed Date:  2006-08-04     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7905558     Medline TA:  J Neurosci Methods     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  230-8     Citation Subset:  IM    
Affiliation:
Laboratory of Animal Physiology, Department of Animal Biology, Faculty of Biology, University of La Laguna, 38206 Tenerife, and Research Centre for Neurological Diseases (CIEN), ISCIII, Spain.
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MeSH Terms
Descriptor/Qualifier:
Analysis of Variance
Animals
Cell Line, Transformed
Cell Survival / drug effects
Dose-Response Relationship, Drug
Fatty Acids, Unsaturated / administration & dosage*
Mice
Neurons / drug effects*,  metabolism
Phospholipids / metabolism*
Chemical
Reg. No./Substance:
0/Fatty Acids, Unsaturated; 0/Phospholipids

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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