Document Detail

Selective polyunsaturated fatty acids enrichment in phospholipids from neuronal-derived cell lines.
MedLine Citation:
PMID:  16337275     Owner:  NLM     Status:  MEDLINE    
Most studies aimed at exploring the molecular and cellular properties of plasma membranes in neural tissues make use of cell lines. However, cell membrane lipid composition of cell lines is notably different from that of brain tissues where they presumably derive from. Using septal-derived SN56 cells and hippocampal-derived HT22 cells, we demonstrated that cell lines exhibit lower contents of saturated (18:0) and long polyunsaturated fatty acids (PUFA; 20:4n-6 and especially 22:6n-3), as well as higher monounsaturated fatty acid contents (mainly 18:1n-9), compared to mouse brain. Also, cell lines exhibited higher contents of sterol esters and lower contents of cholesterol and phospholipids, especially phosphatidylethanolamine and phosphatidylserine. We have also evaluated the effects of different (n-3/n-6) PUFA enrichments on fatty acid and phospholipid contents in these cell lines. Our results show that enrichment of culture medium with 22:6n-3 and 20:4n-6 in a 70/30 proportion during 48 h, using fat-free bovine serum albumin as vehicle, successfully readjusted fatty acid profiles in cell line-polar lipids to values found in natural nerve cells. Interestingly, no differences in cell survival were observed upon enrichment. The generalization of these methodologies would allow a more feasible adaptation of cellular models to the study of in vivo nerve physiology.
Virginia Martín; Eduardo Almansa; Noemí Fabelo; Mario Díaz
Related Documents :
6538135 - Structural and functional alterations of lipid-depleted ascites tumor cells in culture.
7048895 - Determination of ldl receptors on cultured lymphocytes using fluorescein-labeled ldl.
8576635 - Effect of a coffee lipid (cafestol) on regulation of lipid metabolism in caco-2 cells.
17172695 - Proteomic analysis of foam cells.
7974515 - Effects of n-acetylcysteine and dithiothreitol on glutathione and protein thiol repleni...
287025 - Migratory patterns of cloned neural crest melanocytes injected into host chicken embryos.
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't     Date:  2005-12-06
Journal Detail:
Title:  Journal of neuroscience methods     Volume:  153     ISSN:  0165-0270     ISO Abbreviation:  J. Neurosci. Methods     Publication Date:  2006 Jun 
Date Detail:
Created Date:  2006-05-15     Completed Date:  2006-08-04     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7905558     Medline TA:  J Neurosci Methods     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  230-8     Citation Subset:  IM    
Laboratory of Animal Physiology, Department of Animal Biology, Faculty of Biology, University of La Laguna, 38206 Tenerife, and Research Centre for Neurological Diseases (CIEN), ISCIII, Spain.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Analysis of Variance
Cell Line, Transformed
Cell Survival / drug effects
Dose-Response Relationship, Drug
Fatty Acids, Unsaturated / administration & dosage*
Neurons / drug effects*,  metabolism
Phospholipids / metabolism*
Reg. No./Substance:
0/Fatty Acids, Unsaturated; 0/Phospholipids

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  An improved method for the separation and detection of biogenic amines in adult Drosophila brain ext...
Next Document:  Analog frontend for multichannel neuronal recording system with spike and LFP separation.