Document Detail

Selective nuclear shrinkage of oligodendrocytes lacking glial cytoplasmic inclusions in multiple system atrophy: a 3-dimensional volumetric study.
MedLine Citation:
PMID:  19918120     Owner:  NLM     Status:  MEDLINE    
Glial cytoplasmic inclusions (GCIs) are a pathologic hallmark of multiple system atrophy (MSA), but their pathogenetic roles need to be clarified. To determine possible roles of GCIs in individual cells, serial optical sections obtained by confocal microscopy were reconstructed to yield 3-dimensional (3D) images of the nuclei to quantify nuclear volume. Oligodendroglial nuclear volumes were determined in the pons of 6 MSA and 7 control patients. The nuclear volumes were significantly smaller in the MSA group as a whole (135.81 +/- 60.83 microm, mean +/- SD; n = 404) than in the control group (188.05 +/- 55.71 microm; n = 308; p < 0.001). This difference was due to a significantly smaller nuclear volume of oligodendrocytes without GCIs (GCI group, 91.26 +/- 23.77 microm; n = 210) compared with the control group (p < 0.001) and compared with the oligodendrocytes with GCIs (GCI group, 184.03 +/- 51.18 microm; n = 194; p < 0.001); the difference between the latter GCI and control groups was not significant (p > 0.05). This selective decrease in nuclear volume restricted to the GCI group cannot be explained if nuclear shrinkage was accelerated in the presence of GCIs. Conversely, GCI formation might be linked, either directly or indirectly, to a mechanism that counteracts rather than accelerates nuclear shrinkage. This novel 3-dimensional strategy provides pivotal data that link GCI formation and degeneration in MSA.
Naoto Uyama; Toshiki Uchihara; Yoko Mochizuki; Ayako Nakamura; Ryosuke Takahashi; Toshio Mizutani
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of neuropathology and experimental neurology     Volume:  68     ISSN:  1554-6578     ISO Abbreviation:  J. Neuropathol. Exp. Neurol.     Publication Date:  2009 Oct 
Date Detail:
Created Date:  2009-11-17     Completed Date:  2009-12-04     Revised Date:  2012-12-07    
Medline Journal Info:
Nlm Unique ID:  2985192R     Medline TA:  J Neuropathol Exp Neurol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1084-91     Citation Subset:  IM    
Department of Neurology, Tokyo Metropolitan Institute for Neuroscience, Tokyo, Japan.
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MeSH Terms
Aged, 80 and over
Analysis of Variance
Cell Nucleus / pathology*
Cell Nucleus Size*
Imaging, Three-Dimensional
Inclusion Bodies / pathology*
Microscopy, Confocal
Microscopy, Fluorescence
Middle Aged
Multiple System Atrophy / pathology*
Oligodendroglia / pathology*
Pons / pathology*

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