Document Detail


Selective measurement and manipulation of adhesion forces between cancer cells and bone marrow endothelial cells using atomic force microscopy.
MedLine Citation:
PMID:  23199365     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Aims: The lack of understanding of the biology of bone cancer metastasis has limited the development of effective treatment strategies. The aim of this study was to characterize tumor cell adhesion molecules and determine active tumor cell interactions with human bone marrow endothelial (BME) cells using atomic force microscopy. Materials & methods: A single prostate (PC3) cancer cell was coupled (concanavalin A) to the atomic force microscopy cantilever then placed in contact with BME cells for cell force spectroscopy measurements. Results & discussion: Strong adhesive interactions between PC3 and BME cells were significantly (p < 0.05) reduced by anti-ICAM-1, anti-β1 and anti-P-selectin, but not anti-VCAM-1. The combined blocking antibodies or the therapeutic agent zoledronic acid significantly (p < 0.005) reduced the adhesive interactions by 65 and 63%, respectively, which was confirmed using a functional in vitro assay. Conclusion: Atomic force microscopy provides a highly sensitive screening assay to determine and quantify nanoscale adhesion events between different cell types important in the metastatic cascade. Original submitted 21 February 2012; Revised submitted 30 July 2012.
Authors:
Kimberley J Reeves; Jia Hou; Susan E Higham; Zhe Sun; Jerome P Trzeciakowski; Gerald A Meininger; Nicola J Brown
Related Documents :
23121235 - Thermoresponsive cell culture substrates based on pnipam brushes functionalized with ad...
24634585 - Direct role of interrod spacing in mediating cell adhesion on sr-ha nanorod-patterned c...
3934435 - A scanning and transmission electron microscopic examination of rickettsia tsutsugamush...
24311785 - α snap regulates matrix adhesion and integrin processing in human epithelial cells.
25410365 - Targeting of ecm molecules and their metabolizing enzymes and receptors for the treatme...
14635805 - Histochemical analysis of lymphatic endothelial cells in the pancreas of non-obese diab...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-12-2
Journal Detail:
Title:  Nanomedicine (London, England)     Volume:  -     ISSN:  1748-6963     ISO Abbreviation:  Nanomedicine (Lond)     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-12-3     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101278111     Medline TA:  Nanomedicine (Lond)     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Microcirculation Research Group, Department of Oncology, School of Medicine, University of Sheffield, S10 2RX, UK.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Utility of oral cancer diagnostic adjuncts in the adult US populations.
Next Document:  Assessment of immunotoxicity using precision-cut tissue slices.