Document Detail


Selective measurement and manipulation of adhesion forces between cancer cells and bone marrow endothelial cells using atomic force microscopy.
MedLine Citation:
PMID:  23199365     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Aims: The lack of understanding of the biology of bone cancer metastasis has limited the development of effective treatment strategies. The aim of this study was to characterize tumor cell adhesion molecules and determine active tumor cell interactions with human bone marrow endothelial (BME) cells using atomic force microscopy. Materials & methods: A single prostate (PC3) cancer cell was coupled (concanavalin A) to the atomic force microscopy cantilever then placed in contact with BME cells for cell force spectroscopy measurements. Results & discussion: Strong adhesive interactions between PC3 and BME cells were significantly (p < 0.05) reduced by anti-ICAM-1, anti-β1 and anti-P-selectin, but not anti-VCAM-1. The combined blocking antibodies or the therapeutic agent zoledronic acid significantly (p < 0.005) reduced the adhesive interactions by 65 and 63%, respectively, which was confirmed using a functional in vitro assay. Conclusion: Atomic force microscopy provides a highly sensitive screening assay to determine and quantify nanoscale adhesion events between different cell types important in the metastatic cascade. Original submitted 21 February 2012; Revised submitted 30 July 2012.
Authors:
Kimberley J Reeves; Jia Hou; Susan E Higham; Zhe Sun; Jerome P Trzeciakowski; Gerald A Meininger; Nicola J Brown
Related Documents :
22981075 - Comparison of endothelialization and neointimal formation with stents coated with antib...
23900705 - Patterned cell culture substrates created by hot embossing of tissue culture treated po...
23037715 - Ligand-independent activation of epha2 by arachidonic acid induces metastasis-like beha...
23417915 - Glycosylated nanoscale surfaces: preparation and applications in medicine and molecular...
7686555 - Peptides derived from two separate domains of the matrix protein thrombospondin-1 have ...
22981075 - Comparison of endothelialization and neointimal formation with stents coated with antib...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-12-2
Journal Detail:
Title:  Nanomedicine (London, England)     Volume:  -     ISSN:  1748-6963     ISO Abbreviation:  Nanomedicine (Lond)     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-12-3     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101278111     Medline TA:  Nanomedicine (Lond)     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Microcirculation Research Group, Department of Oncology, School of Medicine, University of Sheffield, S10 2RX, UK.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Utility of oral cancer diagnostic adjuncts in the adult US populations.
Next Document:  Assessment of immunotoxicity using precision-cut tissue slices.