Document Detail


Selective ligation of DNA molecules following microinjection.
MedLine Citation:
PMID:  6311814     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Avian retroviral DNA is rapidly ligated and transcribed following microinjection into chick cells. To analyze the fidelity of intranuclear ligations, DNA molecules with a variety of protruding ends or flush ends were co-injected into env-deficient Rous sarcoma virus-infected (RSV(-] cells. Two fragments were chosen which could complement the RSV(-) env deficiency upon ligation. This sensitive biological assay indicated that intracellular ligation between molecules with complementary protruding ends or flush ends was relatively efficient. Ligation was not observed with non-complementary termini. Furthermore, when multiple DNA molecules possessing a variety of protruding termini were introduced into the cells, complementary termini were selectively joined. Finally, when viral DNAs which expressed separate genetic markers were co-injected, almost 50% of the virus subsequently released were recombinants, indicating that ligations generally involved multiple molecules.
Authors:
J J Kopchick; D W Stacey
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  258     ISSN:  0021-9258     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  1983 Oct 
Date Detail:
Created Date:  1983-11-23     Completed Date:  1983-11-23     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  11528-36     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Avian Sarcoma Viruses / genetics*
Base Sequence
Cell Line
Cell Transformation, Neoplastic*
DNA Restriction Enzymes
DNA, Recombinant / metabolism
DNA, Viral / genetics*,  isolation & purification
Genetic Complementation Test
Microinjections
Plasmids
Transcription, Genetic*
Chemical
Reg. No./Substance:
0/DNA, Recombinant; 0/DNA, Viral; EC 3.1.21.-/DNA Restriction Enzymes

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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