| Selective ligand-induced intracellular calcium changes in a population of rat isolated gastric endocrine cells. | |
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MedLine Citation:
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PMID: 8964409 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND & AIMS: Peripheral regulation of acid secretion depends mainly on stimulation or inhibition of the three major gastric endocrine cells (enterochromaffin-like, gastrin, and somatostatin). The aim of this paper was to define physiological responses of enterochromaffin-like, gastrin, and somatostatin cells in a mixed endocrine cell population by measuring ligand-selective changes of intracellular calcium ([Ca2+]i) in individual cells. METHODS: Endocrine cells were enriched from a rat gastric cell suspension by elutriation, a density-gradient fractionation, and a 48-hour short-term culture. [Ca2+]i responses of individual cells to various ligands such as gastrin/carboxy-terminal cholecystokinin octapeptide and selective cholecystokinin antagonists, carbachol, and gastrin-releasing peptide were monitored using video imaging in a perfusion chamber. Characteristic [Ca2+]i changes distinguished the three cell types, confirmed by immunostaining. RESULTS: All enterochromaffin-like cells respond to cholecystokinin-B receptor stimulation, but only a few respond to carbachol. Gastrin cells respond to both gastrin-releasing peptide and carbachol but not to cholecystokinin-receptor agonists. Somatostatin cells have both stimulatory cholecystokinin-A and cholecystokinin-B receptors and inhibitory muscarinic receptors. All cells have inhibitory somatostatin receptors. CONCLUSIONS: Calcium-signaling responses of gastric endocrine cells are distinctive. This allows individual cell types in a mixed population to be characterized and permits an analysis of the hormones and transmitters that act directly on a specific cell type. |
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Authors:
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N Zeng; J H Walsh; T Kang; K G Helander; H F Helander; G Sachs |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Gastroenterology Volume: 110 ISSN: 0016-5085 ISO Abbreviation: Gastroenterology Publication Date: 1996 Jun |
Date Detail:
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Created Date: 1996-12-12 Completed Date: 1996-12-12 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0374630 Medline TA: Gastroenterology Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1835-46 Citation Subset: AIM; IM |
Affiliation:
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Department of Physiology and Medicine, UCLA, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Calcium / metabolism* Chromaffin System / cytology, metabolism Endocrine Glands / cytology, metabolism* Female Intracellular Membranes / metabolism* Ligands Rats Rats, Sprague-Dawley Receptors, Bombesin / metabolism Receptors, Cholecystokinin / metabolism Receptors, Muscarinic / metabolism Receptors, Somatostatin / metabolism Stomach / cytology, metabolism* |
| Grant Support | |
ID/Acronym/Agency:
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DK17294/DK/NIDDK NIH HHS; DK40615/DK/NIDDK NIH HHS; DK41301/DK/NIDDK NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Ligands; 0/Receptors, Bombesin; 0/Receptors, Cholecystokinin; 0/Receptors, Muscarinic; 0/Receptors, Somatostatin; 7440-70-2/Calcium |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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