| Selective labelling of hepatic fatty acids in vivo. Studies on the synthesis and secretion of glycerolipids in the rat. | |
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MedLine Citation:
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PMID: 1567362 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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1. We describe a method for the selective labelling of hepatic fatty acids in the rat in vivo. It relies on (i) the rapid and preferential uptake of cholesteryl ester from chylomicron and/or very-low-density-lipoprotein remnants by the liver [Holder, Zammit & Robinson (1990) Biochem. J. 272, 735-741] (without prior exchange of the ester to other lipoproteins in the plasma), and (ii) the very short half-life of the cholesteryl ester in the liver. The 14C-labelled fatty acid moiety generated by cholesteryl ester hydrolysis was shown to be utilized by the liver for glycerolipid synthesis in a very similar pattern to that demonstrated for exogenous fatty acids by isolated cultured hepatocytes in previous studies. 2. Starvation (24 h) was shown to decrease the proportion of fatty acid utilized for glycerolipid synthesis, but to result in a proportionately smaller effect on incorporation into phospholipid. This was accompanied by a decrease in the fraction of synthesized triacylglycerol that was secreted by the liver. 3. Streptozotocin-diabetes did not affect the phospholipid/triacylglycerol ratio, but resulted in a small, but significant, decline in the fraction of triacylglycerol secreted by the liver. 4. In both starved and diabetic animals fatty acid esterification to the glycerol moiety constituted a smaller proportion of the total disposal of label. 5. These findings appear to validate the present method for the selective labelling of liver fatty acids in vivo in a non-invasive manner. Other possible uses for the method are suggested. |
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Authors:
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A M Moir; V A Zammit |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The Biochemical journal Volume: 283 ( Pt 1) ISSN: 0264-6021 ISO Abbreviation: Biochem. J. Publication Date: 1992 Apr |
Date Detail:
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Created Date: 1992-05-19 Completed Date: 1992-05-19 Revised Date: 2010-09-07 |
Medline Journal Info:
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Nlm Unique ID: 2984726R Medline TA: Biochem J Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 145-9 Citation Subset: IM |
Affiliation:
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Hannah Research Institute, Ayr, Scotland, U.K. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Apolipoproteins C / metabolism, pharmacokinetics Apolipoproteins E / metabolism, pharmacokinetics Carbon Radioisotopes Cholesterol Esters / metabolism, pharmacokinetics Fatty Acids / metabolism*, secretion Half-Life Hydrolysis Lipids / biosynthesis, secretion Lipoproteins, VLDL / pharmacokinetics Liver / metabolism*, secretion Male Phospholipids / metabolism Rats Rats, Inbred Strains Triglycerides / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Apolipoproteins C; 0/Apolipoproteins E; 0/Carbon Radioisotopes; 0/Cholesterol Esters; 0/Fatty Acids; 0/Lipids; 0/Lipoproteins, VLDL; 0/Phospholipids; 0/Triglycerides; 303-43-5/cholesteryl oleate |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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