Document Detail


Selective inhibitory effect of bufalin on growth of human tumor cells in vitro: association with the induction of apoptosis in leukemia HL-60 cells.
MedLine Citation:
PMID:  8063619     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We found that bufalin, an active principle of the Chinese medicine chan'su, has selective inhibitory effects on the growth of various human cancer cells. In order to examine whether the growth-inhibitory effect of bufalin on human cancer cells is associated with apoptosis, human leukemia cells were treated with bufalin. HL-60, ML1, and U937 leukemia cells treated with bufalin at 10(-8) M and above had condensed and fragmented nuclei. Flow cytometric analysis of these cells treated with bufalin showed fragmented DNA smaller than that of the G1 phase. DNA of HL-60 cells treated with bufalin showed a ladder pattern characteristic of apoptosis, as analyzed by agarose gel electrophoretic analysis. DNA synthesis and topoisomerase II activity of HL-60 cells were markedly inhibited as the concentration of bufalin was increased. The concentration needed for inducing apoptosis of HL-60 cells was 10(-8) M, which is comparable to that of camptothecin, but lower than those of other antitumor drugs such as cisplatin, VP16 and all-trans retinoic acid. Apoptosis was not observed when human mononuclear and polymorphonuclear cells were treated with 10(-6) M bufalin for 24 h. These results indicate the association of the growth-inhibitory effect of bufalin with the induction of apoptosis, at least in HL-60 cells, and suggest the usefulness of bufalin for differentiation-apoptosis-inducing therapy for cancer.
Authors:
Y Jing; H Ohizumi; N Kawazoe; S Hashimoto; Y Masuda; S Nakajo; T Yoshida; Y Kuroiwa; K Nakaya
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Japanese journal of cancer research : Gann     Volume:  85     ISSN:  0910-5050     ISO Abbreviation:  Jpn. J. Cancer Res.     Publication Date:  1994 Jun 
Date Detail:
Created Date:  1994-09-21     Completed Date:  1994-09-21     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  8509412     Medline TA:  Jpn J Cancer Res     Country:  JAPAN    
Other Details:
Languages:  eng     Pagination:  645-51     Citation Subset:  IM    
Affiliation:
Department of Biological Chemistry, School of Pharmaceutical Sciences, Showa University, Tokyo.
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MeSH Terms
Descriptor/Qualifier:
Antineoplastic Agents / pharmacology*
Apoptosis / drug effects*
Bufanolides / pharmacology*
Cell Death / drug effects
Cell Division / drug effects
DNA, Neoplasm / analysis,  biosynthesis
Electrophoresis, Agar Gel
Hela Cells
Humans
Kinetics
Leukemia, Myeloid / drug therapy*,  metabolism,  pathology
Leukocytes, Mononuclear / chemistry,  metabolism
Materia Medica / pharmacology*
Neoplasm Proteins / biosynthesis
Neutrophils / chemistry,  metabolism
RNA, Neoplasm / biosynthesis
Tumor Cells, Cultured / drug effects
Chemical
Reg. No./Substance:
0/Antineoplastic Agents; 0/Bufanolides; 0/DNA, Neoplasm; 0/Materia Medica; 0/Neoplasm Proteins; 0/RNA, Neoplasm; 465-21-4/bufalin

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