Document Detail

Selective inhibition of bleomycin-induced G2 cell cycle checkpoint by simaomicin alpha.
MedLine Citation:
PMID:  15081413     Owner:  NLM     Status:  MEDLINE    
Human T-cell leukemia-derived Jurkat cells are known to be defective in the G1 checkpoint. DNA-damaging agent bleomycin arrests the cell cycle at G2 phase of Jurkat cells, and microtubule-acting colchicine arrests it at the M phase. Simaomicin alpha, an actinomycete metabolite, itself showed no effect on the cell cycle status of Jurkat cells at least up to 6.0 nM. However, the compound (0.6-6.0 nM) was found to abrogate the bleomycin-induced G2 arrest, yielding a drastic decrease in cells at the G2 phase and increase in cells at the subG1 and G1 phases. On the other hand, the compound did not show any effect on the colchicine-induced M phase arrest in Jurkat cells. Furthermore, the compound showed almost no effect on the cell cycle status of the bleomycin-treated or -untreated normal cell line HUVEC. These data suggested that simaomicin alpha disrupts the cell cycle G2 checkpoint of cancer cells selectively, leading to sensitization of cancer cells to anti-cancer reagents.
Masayoshi Arai; Hitoshi Sato; Hidetaka Kobayashi; Masashi Suganuma; Takumi Kawabe; Hiroshi Tomoda; Satoshi Omura
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  317     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  2004 May 
Date Detail:
Created Date:  2004-04-14     Completed Date:  2004-05-19     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  817-22     Citation Subset:  IM    
Kitasato Institute for Life Sciences and Graduate School of Infection Control Sciences, Kitasato University, 5-9-1 Shirokane Minato-ku, Tokyo 108-8641, Japan.
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MeSH Terms
Bleomycin / antagonists & inhibitors*,  pharmacology
G2 Phase / drug effects*
Isoquinolines / pharmacology*
Jurkat Cells
Reg. No./Substance:
0/Isoquinolines; 100157-22-0/simaomicin alpha; 11056-06-7/Bleomycin

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