Document Detail


Selective induction of apoptosis in glioma tumour cells by a Gynostemma pentaphyllum extract.
MedLine Citation:
PMID:  20106643     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
At low concentration H(2)O(2) is an important signal molecule in proliferation of tumour cells. We report about a study investigating the effect of an ethanolic extract from Gynostemma pentaphyllum on proliferation of C6 glioma tumour cells and cellular H(2)O(2) concentration. The proliferation of these cells was maximal at about 1 muM extracellular H(2)O(2). HPLC-finger prints of the extract revealed a set of saponines as essential components. In C6 glioma cells the extract caused increase in super oxide dismutase (SOD) activity, in the amount of SOD protein, and in cellular H(2)O(2) concentration. It inhibited cell proliferation and induced activation of caspase 3 as indication of apoptosis. No effect of the extract was observed on the proliferation of astrocytes of a primary cell culture. From these findings we suggest that the ethanolic extract from Gynostemma pentaphyllum may selectively shift the H(2)O(2) concentration to toxic levels exclusively in tumour cells due to increased SOD activity. It may have a high potency in cancer therapy and cancer prophylaxis.
Authors:
L Schild; B H Chen; P Makarov; K Kattengell; K Heinitz; G Keilhoff
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Publication Detail:
Type:  Journal Article     Date:  2010-01-27
Journal Detail:
Title:  Phytomedicine : international journal of phytotherapy and phytopharmacology     Volume:  17     ISSN:  1618-095X     ISO Abbreviation:  Phytomedicine     Publication Date:  2010 Jul 
Date Detail:
Created Date:  2010-05-24     Completed Date:  2010-12-03     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9438794     Medline TA:  Phytomedicine     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  589-97     Citation Subset:  IM    
Copyright Information:
(c) 2010 Elsevier GmbH. All rights reserved.
Affiliation:
Department of Pathological Biochemistry, Otto-von-Guericke University, Magdeburg, Germany. lorenz.schild@med.ovgu.de
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MeSH Terms
Descriptor/Qualifier:
Animals
Antineoplastic Agents, Phytogenic / pharmacology,  therapeutic use*
Apoptosis / drug effects*
Astrocytes / drug effects
Brain Neoplasms / drug therapy*,  metabolism
Caspase 3 / metabolism
Cell Line
Cell Line, Tumor
Cell Proliferation / drug effects
Chromatography, High Pressure Liquid
Glioma / drug therapy*,  metabolism
Gynostemma / chemistry*
Humans
Hydrogen Peroxide / metabolism*
Phytotherapy
Plant Extracts / chemistry,  pharmacology,  therapeutic use*
Rats
Rats, Wistar
Saponins / analysis,  pharmacology,  therapeutic use
Signal Transduction / drug effects
Superoxide Dismutase / metabolism
Chemical
Reg. No./Substance:
0/Antineoplastic Agents, Phytogenic; 0/Plant Extracts; 0/Saponins; 7722-84-1/Hydrogen Peroxide; EC 1.15.1.1/Superoxide Dismutase; EC 3.4.22.-/Caspase 3

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