| Selective induction of apoptosis in glioma tumour cells by a Gynostemma pentaphyllum extract. | |
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MedLine Citation:
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PMID: 20106643 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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At low concentration H(2)O(2) is an important signal molecule in proliferation of tumour cells. We report about a study investigating the effect of an ethanolic extract from Gynostemma pentaphyllum on proliferation of C6 glioma tumour cells and cellular H(2)O(2) concentration. The proliferation of these cells was maximal at about 1 muM extracellular H(2)O(2). HPLC-finger prints of the extract revealed a set of saponines as essential components. In C6 glioma cells the extract caused increase in super oxide dismutase (SOD) activity, in the amount of SOD protein, and in cellular H(2)O(2) concentration. It inhibited cell proliferation and induced activation of caspase 3 as indication of apoptosis. No effect of the extract was observed on the proliferation of astrocytes of a primary cell culture. From these findings we suggest that the ethanolic extract from Gynostemma pentaphyllum may selectively shift the H(2)O(2) concentration to toxic levels exclusively in tumour cells due to increased SOD activity. It may have a high potency in cancer therapy and cancer prophylaxis. |
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Authors:
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L Schild; B H Chen; P Makarov; K Kattengell; K Heinitz; G Keilhoff |
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Publication Detail:
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Type: Journal Article Date: 2010-01-27 |
Journal Detail:
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Title: Phytomedicine : international journal of phytotherapy and phytopharmacology Volume: 17 ISSN: 1618-095X ISO Abbreviation: Phytomedicine Publication Date: 2010 Jul |
Date Detail:
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Created Date: 2010-05-24 Completed Date: 2010-12-03 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9438794 Medline TA: Phytomedicine Country: Germany |
Other Details:
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Languages: eng Pagination: 589-97 Citation Subset: IM |
Copyright Information:
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(c) 2010 Elsevier GmbH. All rights reserved. |
Affiliation:
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Department of Pathological Biochemistry, Otto-von-Guericke University, Magdeburg, Germany. lorenz.schild@med.ovgu.de |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antineoplastic Agents, Phytogenic / pharmacology, therapeutic use* Apoptosis / drug effects* Astrocytes / drug effects Brain Neoplasms / drug therapy*, metabolism Caspase 3 / metabolism Cell Line Cell Line, Tumor Cell Proliferation / drug effects Chromatography, High Pressure Liquid Glioma / drug therapy*, metabolism Gynostemma / chemistry* Humans Hydrogen Peroxide / metabolism* Phytotherapy Plant Extracts / chemistry, pharmacology, therapeutic use* Rats Rats, Wistar Saponins / analysis, pharmacology, therapeutic use Signal Transduction / drug effects Superoxide Dismutase / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Antineoplastic Agents, Phytogenic; 0/Plant Extracts; 0/Saponins; 7722-84-1/Hydrogen Peroxide; EC 1.15.1.1/Superoxide Dismutase; EC 3.4.22.-/Caspase 3 |
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