Document Detail


Selective induction of apoptosis of NB4 cells from G2+M phase by sodium arsenite at lower doses.
MedLine Citation:
PMID:  9688289     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Apoptosis of NB4 cells induced by sodium arsenite and arsenate was studied using flow cytometry and DNA gel electrophoresis in order to investigate their effects on cell cycle and determine the relationship between apoptosis and cell cycle. In this study, we found that: 1) at low doses, sodium arsenite selectively induced apoptosis of NB4 cells in G2+M phase of cell cycle after its being arrested in G2 phase. With increment of the cells blocked in G2 phase, dUTP-specifically labeling cells in G2+M phase increased without concomitant increment of dUTP-labeling cells in other two phases of cell cycle; 2) at high doses, extensive apoptosis was induced in NB4 cells from all phases of cell cycle without cell cycle preference and cell cycle blockade; 3) sodium arsenite-induced apoptosis of NB4 cells occurred in the presence of bcl-2 expression as the unapoptotic cells; 4) sodium arsenite with As3+ induced apoptosis of NB4 cells more strongly than sodium arsenate with As5+ did although both of them affected NB4 cells in the same pattern. These results not only suggested that both arsenite and arsenate induced apoptosis of NB4 cells through 2 different mechanisms--at low doses, arsenical might directly induce apoptosis through regulation of cell cycle checkpoint, while at high doses they might directly induce it, but also indicated that bcl-2 might not play an important role in arsenite or arsenate-induced apoptosis of NB4 cells, whereas chemical valence of As in a compound might be related to efficiency in arsenical induction of apoptosis.
Authors:
D C Ma; Y H Sun; K Z Chang; X F Ma; S L Huang; Y H Bai; J Kang; Y G Liu; J J Chu
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  European journal of haematology     Volume:  61     ISSN:  0902-4441     ISO Abbreviation:  Eur. J. Haematol.     Publication Date:  1998 Jul 
Date Detail:
Created Date:  1998-09-01     Completed Date:  1998-09-01     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  8703985     Medline TA:  Eur J Haematol     Country:  DENMARK    
Other Details:
Languages:  eng     Pagination:  27-35     Citation Subset:  IM    
Affiliation:
Department of Experimental Medicine, Northern Hospital, Shenyang, Liaoning, China.
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MeSH Terms
Descriptor/Qualifier:
Apoptosis / drug effects*
Arsenates / pharmacology
Arsenites / pharmacology*
Cell Division / drug effects
Dose-Response Relationship, Drug
Flow Cytometry
G2 Phase / drug effects*
Humans
Mitosis / drug effects*
Proto-Oncogene Proteins c-bcl-2 / biosynthesis
Tumor Cells, Cultured / cytology
Chemical
Reg. No./Substance:
0/Arsenates; 0/Arsenites; 0/Proto-Oncogene Proteins c-bcl-2

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