Document Detail


Selective impairment of vagally mediated, nitric oxide-dependent coronary vasodilation in conscious dogs after pacing-induced heart failure.
MedLine Citation:
PMID:  7743629     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Activation in conscious dogs of the carotid chemoreflex or cardiac receptors results in coronary vasodilation that is mediated by a vagal cholinergic mechanism. Our previous study showed that the coronary vasodilation following activation of carotid chemoreflex is also mediated by nitric oxide (NO). In addition, NO production is depressed after the development of heart failure. Therefore, we hypothesized that the coronary vasodilation after activation of reflexes that elicit efferent vagal coronary vasodilation would be blunted in conscious dogs after pacing-induced heart failure due to the disappearance of NO. METHODS AND RESULTS: Mongrel dogs were chronically instrumented using sterile techniques for measurements of systemic hemodynamics and left circumflex coronary blood flow (CBF). Without the heart rate controlled, intra-atrial injection of veratrine (4 micrograms/kg) caused bradycardia (-36 +/- 3 beats per minute). With the heart rate controlled, veratrine increased CBF in a dose-dependent manner: for example, 4 micrograms/kg of veratrine increased CBF by 54 +/- 5% from 38 +/- 4.9 mL/min (P < .05). The increases in CBF induced by veratrine were markedly blunted by nitro-L-arginine (NLA). Activation of carotid chemoreflex by nicotine increased CBF by 121 +/- 9% from 32 +/- 4 mL++/min (P < .05) with the heart rate controlled and caused bradycardia (-32 +/- 5 beats per minute) without the heart rate controlled. After the development of heart failure, in response to activation of carotid chemoreflex or cardiac receptors the coronary vasodilation was almost abolished (CBF increased by only 23 +/- 8% or 11 +/- 3%, P < .05 compared with control). There still was a marked bradycardia after injections of nicotine or veratrine (-50 +/- 11 or -48 +/- 7 beats per minute). CONCLUSIONS: Our results indicate that vagally mediated coronary vasodilation is selectively attenuated in conscious dogs after pacing-induced heart failure, whereas the vagally mediated bradycardia is preserved. Since muscarinic receptor-induced coronary vasodilation is mediated by NO, the disappearance of NO from blood vessels leads to a defect in the integrated neural regulation of coronary blood flow and myocardial function during heart failure.
Authors:
G Zhao; W Shen; X Xu; M Ochoa; R Bernstein; T H Hintze
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Circulation     Volume:  91     ISSN:  0009-7322     ISO Abbreviation:  Circulation     Publication Date:  1995 May 
Date Detail:
Created Date:  1995-06-15     Completed Date:  1995-06-15     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0147763     Medline TA:  Circulation     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  2655-63     Citation Subset:  AIM; IM    
Affiliation:
Department of Physiology, New York Medical College, Valhalla 10595, USA.
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MeSH Terms
Descriptor/Qualifier:
Acetylcholine / pharmacology
Adenosine / pharmacology
Animals
Arginine / analogs & derivatives,  pharmacology
Cardiac Output, Low / etiology,  physiopathology*
Cardiac Pacing, Artificial
Chemoreceptor Cells / drug effects,  physiology
Coronary Circulation / drug effects,  physiology*
Dogs
Heart Rate
Hemodynamics / drug effects
Nicotine / pharmacology
Nitric Oxide / physiology*
Nitroarginine
Reflex / physiology
Vagus Nerve / physiopathology*
Vasodilation / physiology*
Veratrine / pharmacology
Grant Support
ID/Acronym/Agency:
P0-1-HL-43023/HL/NHLBI NIH HHS; R0-1-50142//PHS HHS; R0-1-53053//PHS HHS
Chemical
Reg. No./Substance:
10102-43-9/Nitric Oxide; 2149-70-4/Nitroarginine; 51-84-3/Acetylcholine; 54-11-5/Nicotine; 58-61-7/Adenosine; 62-59-9/Veratrine; 74-79-3/Arginine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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