Document Detail

Selective imaging of saturated and unsaturated lipids by wide-field CARS-microscopy.
MedLine Citation:
PMID:  18542355     Owner:  NLM     Status:  MEDLINE    
Wide-field Coherent Anti-Stokes Raman Scattering (CARS) microscopy is employed to identify saturated and unsaturated fatty acids in micro-emulsions and cells, using the ratio between the strong -C-H CARS signal at 2850 cm(-1) and the weak signal of the =C-H vibration around 3015 cm(-1) for distinction. Quantitative CARS imaging at the =C-H resonance is challenging, since it yields only a low CARS signal, and small differences on the order of 5% in the concentration of polyunsaturated fatty lipids have to be detected. For this purpose we draw advantage of the high signal-to-noise ratio of wide-field CARS microscopy that is achieved by an excitation geometry involving a "sheet-of-light"-type illumination.
Christoph Heinrich; Alexander Hofer; Andreas Ritsch; Christian Ciardi; Stefan Bernet; Monika Ritsch-Marte
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Optics express     Volume:  16     ISSN:  1094-4087     ISO Abbreviation:  Opt Express     Publication Date:  2008 Feb 
Date Detail:
Created Date:  2008-06-10     Completed Date:  2008-07-23     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101137103     Medline TA:  Opt Express     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2699-708     Citation Subset:  IM    
Division for Biomedical Physics, Innsbruck Medical University, Müllerstr. 44, A-6020 Innsbruck, Austria.
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MeSH Terms
Fats, Unsaturated / chemistry
Fatty Acids / chemistry
Lipids / chemistry*
Microscopy / methods
Plant Oils / chemistry
Spectrum Analysis, Raman
Reg. No./Substance:
0/Fats, Unsaturated; 0/Fatty Acids; 0/Lipids; 0/Plant Oils

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