Document Detail


Selective fetal hypophysectomy in utero: microsurgical technique and comparisons with hypophysectomy by decapitation.
MedLine Citation:
PMID:  12673099     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A detailed description is given of a surgical technique for selectively removing the pituitary anlage from the intrauterine fetal rat as an alternative to the more generally applied fetal hypophysectomy by decapitation. Fetuses on the 17th day of gestation were approached through a narrow opening in the uterine wall and fetal membranes. The pituitary gland was sucked out with a fine glass tube introduced from the side of the head into the space between the pons and the ventral hypothalamus, where the gland is situated. The female rat was sacrificed on the 22nd day of gestation (the day before delivery) and the surviving fetuses were preserved. On average, 20% of the operated fetuses survived until that day, 20% of which were completely hypophysectomized (microscopical examination). Operated fetuses with intact pituitaries served as surgical controls for the evaluation of selected effects of the total hypophysectomy. Initial comparisons were made with published results based on hypophysectomy by decapitation, evaluated without access to surgical controls. The two methods of fetal pituitary removal provide consistent results as regards the weight increase of adrenal and thyroid glands (retardation), thymus gland (stimulation) and testicles (no effect). On the other hand, body weight increase was not affected by selective hypophysectomy but instead slowed down after decapitation, whereas the growth in length of the humerus bone was stimulated and retarded, respectively.
Authors:
Anders Enemar
Related Documents :
16287959 - Adrenal insufficiency.
6133799 - Remission of cushing's syndrome during pregnancy.
16429419 - Pattern of maternal serum corticotropin-releasing hormone concentration during pregnanc...
10692849 - Control of parturition in ruminants.
17261319 - Response to lps in female offspring from sows treated with cortisol during pregnancy.
24423279 - Factors affecting uptake of optimal doses of sulphadoxine-pyrimethamine for intermitten...
20070939 - Quantifying the risk of pandemic influenza in pregnancy and indigenous people in austra...
21988789 - Induced abortion in tehran, iran: estimated rates and correlates.
15167819 - Effect of pregnancy and stage of pregnancy on asthma severity: a systematic review.
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cells, tissues, organs     Volume:  173     ISSN:  1422-6405     ISO Abbreviation:  Cells Tissues Organs (Print)     Publication Date:  2003  
Date Detail:
Created Date:  2003-04-03     Completed Date:  2003-10-29     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  100883360     Medline TA:  Cells Tissues Organs     Country:  Switzerland    
Other Details:
Languages:  eng     Pagination:  172-83     Citation Subset:  IM    
Copyright Information:
Copyright 2003 S. Karger AG, Basel
Affiliation:
Department of Zoology, University of Göteborg, Göteborg, Sweden. anders.enemar@zool.gu.se
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Body Weight
Endocrine Glands / embryology
Female
Fetus / anatomy & histology,  surgery*
Gestational Age
Humerus / embryology
Hypophysectomy / methods*
Male
Organ Size
Pituitary Gland / embryology*
Rats
Rats, Sprague-Dawley
Testis / embryology

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Effect of altered thyroid state on the in situ mechanical properties of adult cat soleus.
Next Document:  Immunolocalization of fibronectin and collagen types I and III in human fetal parotid and submandibu...