Document Detail


Selective extraction of organophosphorus nerve agent degradation products by molecularly imprinted solid-phase extraction.
MedLine Citation:
PMID:  16451804     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The analysis of alkyl alkylphosphonic acids, the degradation products of V and G nerve agents as VX, Sarin or Soman, is an important task for the verification of compliance to the Chemical Weapons Convention. The detection of these contaminants at low concentration levels is often difficult in complex matrices due to the amount of interfering substances. Molecularly imprinted solid-phase extraction technique should allow a selective extraction of these compounds from complex samples, and thus make their detection easier. Two molecularly imprinted polymers (MIPs) prepared with methacrylic acid (MAA) as monomer and pinacolyl methylphosphonic acid (PMPA) as template molecule were synthesised and tested. The first polymer, MIP A, was prepared with ethylene glycol dimethacrylate (EGDMA) in dichloromethane. The second polymer, MIP B, was synthesised using trimethylolpropane trimethacrylate (TRIM) in acetonitrile. To evaluate the selectivity provided by these MIPs, the retention of the ethyl methylphosphonic acid (EMPA) target molecule was studied in parallel on a non-imprinted polymer (NIP). While MIP A does not show any difference compared to NIP A, a good selectivity was obtained for MIP B. After the optimisation of the extraction process, 60% of EMPA can be removed from the NIP B without affecting the retention on the MIP B. A recovery of extraction of 93% was then obtained on the MIP B. Its capacity was then measured and corresponds to 97 microg of EMPA per gram of MIP. Finally, the selectivity of MIP B was clearly demonstrated by applying it to the clean-up of a soil extract spiked with EMPA.
Authors:
Sophie Le Moullec; Arlette Bégos; Valérie Pichon; Bruno Bellier
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Publication Detail:
Type:  Evaluation Studies; Journal Article     Date:  2006-01-31
Journal Detail:
Title:  Journal of chromatography. A     Volume:  1108     ISSN:  0021-9673     ISO Abbreviation:  J Chromatogr A     Publication Date:  2006 Mar 
Date Detail:
Created Date:  2006-02-13     Completed Date:  2006-04-17     Revised Date:  2009-01-15    
Medline Journal Info:
Nlm Unique ID:  9318488     Medline TA:  J Chromatogr A     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  7-13     Citation Subset:  IM    
Affiliation:
Département Analyse Chimique, Centre d'études du Bouchet, B.P. 3, 91710 Vert-Le-Petit, France.
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MeSH Terms
Descriptor/Qualifier:
Chemical Fractionation / methods*
Chemical Warfare Agents / isolation & purification*
Methacrylates / chemistry
Organophosphorus Compounds / isolation & purification*
Phosphonic Acids / chemistry
Polymers / chemistry
Sensitivity and Specificity
Soil Pollutants / analysis,  isolation & purification
Solvents
Chemical
Reg. No./Substance:
0/Chemical Warfare Agents; 0/Methacrylates; 0/Organophosphorus Compounds; 0/Phosphonic Acids; 0/Polymers; 0/Soil Pollutants; 0/Solvents; 1832-53-7/ethyl methylphosphonic acid; 3290-92-4/trimethylolpropane trimethacrylate

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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