| Selective extraction of the DM-20 brain proteolipid. | |
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MedLine Citation:
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PMID: 3940288 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Brain DM-20 proteolipid was previously shown to be structurally different from the myelin major proteolipid (MMPL). In an attempt to set up a large-scale purification of DM-20, we studied extraction of brain proteolipids with mixtures of methylene chloride containing up to 80% methanol. The CH2Cl2-CH3OH (3:7, vol/vol) mixture is highly selective for the extraction of bovine brain DM-20 compared to MMPL. Purified DM-20 was obtained after chromatography of the extract on methylated Sephadex. |
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Authors:
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G Skalidis; E Trifilieff; B Luu |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of neurochemistry Volume: 46 ISSN: 0022-3042 ISO Abbreviation: J. Neurochem. Publication Date: 1986 Jan |
Date Detail:
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Created Date: 1986-01-21 Completed Date: 1986-01-21 Revised Date: 2008-07-10 |
Medline Journal Info:
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Nlm Unique ID: 2985190R Medline TA: J Neurochem Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 297-9 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Brain / metabolism* Cattle Electrophoresis, Polyacrylamide Gel Methanol / diagnostic use Methylene Chloride / diagnostic use Myelin Proteolipid Protein* Nerve Tissue Proteins* Proteolipids Rats |
| Chemical | |
Reg. No./Substance:
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0/Myelin Proteolipid Protein; 0/Nerve Tissue Proteins; 0/Plp1 protein, rat; 0/Proteolipids; 67-56-1/Methanol; 75-09-2/Methylene Chloride |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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