| Selective extractability of noncollagenous proteins from chicken bone. | |
| | |
MedLine Citation:
|
PMID: 7987738 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Quantitative analyses of a wide variety of different solvents used for the extraction of several of the noncollagenous proteins of fully mineralized chicken bone powder were carried out to compare both the effectiveness of various procedures and the distribution of specific proteins which were solubilized. Extraction procedures included solutions of 6 M guanidine-HCl, pH 7.0, 0.5 M EDTA, pH 7.4, 0.3 N citric acid, 0.3 N HCl, 0.3 N formic acid, and 0.3 N acetic acid. Chelation of calcium ions by EDTA and dissolution of the mineral phase by acid extraction released 95% or more of the total calcium content of the bone powder by 48 hours, guanidine-HCl released less than 20% or less of the total calcium content even when extraction was carried out by 168 hours. Moreover, although guanidine-HCl solubilized a significant amount of collagen as gelatin, essentially none of the phosphoproteins, osteocalcin, or the proteoglycan decorin were solubilized, as detected by immunological techniques. In contrast, extraction of the mineralized bone powder by HCl and formic acid was very efficient in selectively solubilizing osteocalcin and osteopontin, while bone sialoprotein was selectively released by EDTA, and solubilized to a lesser extent by formic acid. Similarly, EDTA selectively removed decorin compared with HCl, formic, acetic, or citric acids. Only small amounts of osteopontin and osteocalcin were detected in the acetic acid extracts. These results provide methods for the selective solubilization of several different major, noncollagenous proteins from mineralized bone which should significantly aid in maximizing the amount of the specific protein recovered, and the ease with which the various proteins can be purified.(ABSTRACT TRUNCATED AT 250 WORDS) |
| | |
Authors:
|
L C Gerstenfeld; M Feng; Y Gotoh; M J Glimcher |
Related Documents
:
|
7492338 - Metabolism of [3-13c]pyruvate and [3-13c]propionate in normal and ischaemic rat heart i... 1284848 - Relationship between citric acid production and accumulation of phytate-degrading enzym... 15224388 - Retardation of setting of plaster of paris by organic acids: understanding the mechanis... 12740548 - Protective influence of experimentally formed salivary pellicle on enamel erosion. an i... 8821118 - Abnormal membrane concentrations of 20 and 22-carbon essential fatty acids: a common li... 3415238 - Degradation of naphthalene-2,6- and naphthalene-1,6-disulfonic acid by a moraxella sp. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
|
Title: Calcified tissue international Volume: 55 ISSN: 0171-967X ISO Abbreviation: Calcif. Tissue Int. Publication Date: 1994 Sep |
Date Detail:
|
Created Date: 1995-01-06 Completed Date: 1995-01-06 Revised Date: 2007-11-14 |
Medline Journal Info:
|
Nlm Unique ID: 7905481 Medline TA: Calcif Tissue Int Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 230-5 Citation Subset: IM |
Affiliation:
|
Department of Orthopaedic Surgery, Harvard Medical School, Children's Hospital, Boston, Massachusetts 02115. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Blotting, Western Bone and Bones / chemistry* Chickens Collagen / analysis Edetic Acid Extracellular Matrix Proteins Minerals / analysis Osteocalcin / analysis Osteopontin Proteoglycans / analysis Sialoglycoproteins / analysis* |
| Grant Support | |
ID/Acronym/Agency:
|
AR34078/AR/NIAMS NIH HHS; AR34081/AR/NIAMS NIH HHS; HD22400/HD/NICHD NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Extracellular Matrix Proteins; 0/Minerals; 0/Proteoglycans; 0/Sialoglycoproteins; 0/decorin; 104982-03-8/Osteocalcin; 106441-73-0/Osteopontin; 60-00-4/Edetic Acid; 9007-34-5/Collagen |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Relationship between bone mineral density of the proximal femur and lumbar spine and quadriceps and ...
Next Document: Energy-dispersive X-ray microanalysis of the bone mineral content in human trabecular bone: a compar...