Document Detail


Selective expression of high molecular weight basic fibroblast growth factor confers a unique phenotype to NIH 3T3 cells.
MedLine Citation:
PMID:  1660309     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The phenotypes of NIH 3T3 cells transfected with basic fibroblast growth factor (bFGF) cDNAs that express only the high molecular weight (HMW) forms of bFGF, the 18-kDa form, or all forms were examined. Cells producing the 18 kDa or all forms of bFGF were transformed at high levels of growth factor expression but were nontransformed at low levels. Cell producing low levels of HMW forms of bFGF were growth impaired when compared with the parental cells. These cells tended to form multinucleated giant cells, did not grow in soft agar, were nontumorigenic, had a normal bFGF receptor number, and had a nontransformed morphology. Cells expressing high levels of HMW bFGFs had a transformed morphology and were tumorigenic. These data suggest a specific functional role for HMWbFGF.
Authors:
N Quarto; D Talarico; R Florkiewicz; D B Rifkin
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Cell regulation     Volume:  2     ISSN:  1044-2030     ISO Abbreviation:  Cell Regul.     Publication Date:  1991 Sep 
Date Detail:
Created Date:  1992-01-16     Completed Date:  1992-01-16     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  9005331     Medline TA:  Cell Regul     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  699-708     Citation Subset:  IM    
Affiliation:
Department of Cell Biology, New York University Medical Center, New York.
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MeSH Terms
Descriptor/Qualifier:
3T3 Cells
Amino Acid Sequence
Animals
Binding Sites
Blotting, Southern
Cell Division*
Cell Transformation, Neoplastic*
Culture Media
DNA / chemistry,  genetics*
Fibroblast Growth Factor 2 / biosynthesis*,  chemistry,  metabolism
Giant Cells / metabolism
Mice
Molecular Sequence Data
Molecular Weight
Phenotype
Receptors, Cell Surface / metabolism*
Receptors, Fibroblast Growth Factor
Transfection
Chemical
Reg. No./Substance:
0/Culture Media; 0/Receptors, Cell Surface; 0/Receptors, Fibroblast Growth Factor; 103107-01-3/Fibroblast Growth Factor 2; 9007-49-2/DNA
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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