Document Detail


Selective editing of Val and Leu methyl groups in high molecular weight protein NMR.
MedLine Citation:
PMID:  22532128     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The development of methyl-TROSY approaches and specific (13)C-(1)H labeling of Ile, Leu and Val methyl groups in highly deuterated proteins has made it possible to study high molecular weight proteins, either alone or in complexes, using solution nuclear magnetic resonance (NMR) spectroscopy. Here we present 2-dimensional (2D) and 3-dimensional (3D) NMR experiments designed to achieve complete separation of the methyl resonances of Val and Leu, labeled using the same precursor, α-ketoisovalerate or acetolactate. The 2D experiment can further select the methyl resonances of Val or Leu based on the C(α) or C(β) chemical shift values of Val or Leu, respectively. In the 3D spectrum, the methyl cross peaks of Val and Leu residues have opposite signs; thus, not only can the residue types be easily distinguished, but the methyl pairs from the same residue can also be identified. The feasibility of this approach, implemented in both 2D and 3D experiments, has been demonstrated on an 82 kDa protein, malate synthase G. The methods developed in this study will reduce resonance overlaps and also facilitate structure-guided resonance assignments.
Authors:
Weidong Hu; Andrew T Namanja; Steven Wong; Yuan Chen
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2012-04-25
Journal Detail:
Title:  Journal of biomolecular NMR     Volume:  53     ISSN:  1573-5001     ISO Abbreviation:  J. Biomol. NMR     Publication Date:  2012 Jun 
Date Detail:
Created Date:  2012-07-03     Completed Date:  2012-10-23     Revised Date:  2014-10-04    
Medline Journal Info:
Nlm Unique ID:  9110829     Medline TA:  J Biomol NMR     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  113-24     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Carbon Isotopes
Escherichia coli Proteins / chemistry
Leucine / chemistry*
Malate Synthase / chemistry
Models, Molecular
Molecular Weight
Nuclear Magnetic Resonance, Biomolecular / methods*
Proteins / chemistry*
Valine / chemistry*
Grant Support
ID/Acronym/Agency:
R01 GM074748/GM/NIGMS NIH HHS; R01 GM086171/GM/NIGMS NIH HHS; R01GM074748/GM/NIGMS NIH HHS; R01GM086171/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Carbon Isotopes; 0/Escherichia coli Proteins; 0/Proteins; EC 2.3.3.9/Malate Synthase; GMW67QNF9C/Leucine; HG18B9YRS7/Valine
Comments/Corrections

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