Document Detail

Selective digestion of human metaphase chromosomes by Alu I restriction endonuclease.
MedLine Citation:
PMID:  6298309     Owner:  NLM     Status:  MEDLINE    
Human cytological preparations have been digested with Alu I restriction endonuclease (Endo R Alu I) and subsequently stained with acridine orange. Metaphase chromosomes treated in this way revealed a clear-cut bright/ dull longitudinal differentiation. It has been postulated that these cytological findings could be related to the selective digestion, by Alu I, of all DNA with the exception of some heterochromatic, possibly satellite, fractions.
R Mezzanotte; L Ferrucci; R Vanni; U Bianchi
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society     Volume:  31     ISSN:  0022-1554     ISO Abbreviation:  J. Histochem. Cytochem.     Publication Date:  1983 Apr 
Date Detail:
Created Date:  1983-04-21     Completed Date:  1983-04-21     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9815334     Medline TA:  J Histochem Cytochem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  553-6     Citation Subset:  IM    
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MeSH Terms
Chromosomes, Human / analysis*
DNA / analysis*
DNA Restriction Enzymes
Deoxyribonucleases, Type II Site-Specific*
Reg. No./Substance:
9007-49-2/DNA; EC 3.1.21.-/DNA Restriction Enzymes; EC 3.1.21.-/endodeoxyribonuclease AluI; EC, Type II Site-Specific

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