Document Detail


Selective differentiation of neural progenitor cells by high-epitope density nanofibers.
MedLine Citation:
PMID:  14739465     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Neural progenitor cells were encapsulated in vitro within a three-dimensional network of nanofibers formed by self-assembly of peptide amphiphile molecules. The self-assembly is triggered by mixing cell suspensions in media with dilute aqueous solutions of the molecules, and cells survive the growth of the nanofibers around them. These nanofibers were designed to present to cells the neurite-promoting laminin epitope IKVAV at nearly van der Waals density. Relative to laminin or soluble peptide, the artificial nanofiber scaffold induced very rapid differentiation of cells into neurons, while discouraging the development of astrocytes. This rapid selective differentiation is linked to the amplification of bioactive epitope presentation to cells by the nanofibers.
Authors:
Gabriel A Silva; Catherine Czeisler; Krista L Niece; Elia Beniash; Daniel A Harrington; John A Kessler; Samuel I Stupp
Related Documents :
20578725 - Zebrafish (danio rerio) endomembrane antiporter similar to a yeast cation/h(+) transpor...
15892715 - Establishment of a kit-negative cell line of melanocyte precursors from mouse neural cr...
16585955 - The alchemy of tendon repair: a primer for the (s)mad scientist.
15843935 - Assessment of cortical maturation with prenatal mri: part ii: abnormalities of cortical...
3126245 - Mitomycin c-treated 3t3/b (3t3/a31) cell feeder layers in hybridoma technology.
12808345 - Different growth properties of neointimal and medial smooth muscle cells in response to...
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.     Date:  2004-01-22
Journal Detail:
Title:  Science (New York, N.Y.)     Volume:  303     ISSN:  1095-9203     ISO Abbreviation:  Science     Publication Date:  2004 Feb 
Date Detail:
Created Date:  2004-02-27     Completed Date:  2004-03-18     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0404511     Medline TA:  Science     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1352-5     Citation Subset:  IM    
Affiliation:
Institute for Bioengineering and Nanoscience in Advanced Medicine, Northwestern University, Chicago, IL 60611, USA. gsilva@ucsd.edu
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Astrocytes / cytology
Cell Differentiation*
Cell Movement
Cell Survival
Cells, Cultured
Diffusion
Epitopes
Glial Fibrillary Acidic Protein / analysis
Hydrogen Bonding
Hydrophobicity
Laminin / administration & dosage,  chemistry,  immunology,  metabolism*
Mice
Nanotechnology*
Neurites / physiology,  ultrastructure
Neurons / cytology*,  physiology
Peptide Fragments / administration & dosage,  chemistry,  metabolism*
Rats
Spinal Cord
Stem Cells / cytology*,  physiology
Tubulin / analysis
Grant Support
ID/Acronym/Agency:
NS20013/NS/NINDS NIH HHS; NS20778/NS/NINDS NIH HHS; NS34758/NS/NINDS NIH HHS
Chemical
Reg. No./Substance:
0/Epitopes; 0/Glial Fibrillary Acidic Protein; 0/Laminin; 0/Peptide Fragments; 0/Tubulin; 131167-89-0/isoleucyl-lysyl-valyl-alanyl-valine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Human De-etiolated-1 regulates c-Jun by assembling a CUL4A ubiquitin ligase.
Next Document:  Oxygen sensing in neuroendocrine cells and other cell types: pheochromocytoma (PC12) cells as an exp...