| Selective determination of native fluorescent bioamines through precolumn derivatization and liquid chromatography using intramolecular fluorescence resonance energy transfer detection. | |
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MedLine Citation:
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PMID: 16448069 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In this paper, we introduce a novel approach for the highly selective and sensitive analysis of native fluorescent bioamines (indoleamines and catecholamines). This method is based on intramolecular fluorescence resonance energy transfer (FRET) detection in a liquid chromatography (LC) system following precolumn derivatization of the bioamines' amino groups. In this detection process, we monitored the FRET from the native fluorescent moieties (donor) to the derivatized fluorophore (acceptor). From a screening study involving 15 fluorescent reagents, we found that o-phthalaldehyde (OPA) generated the FRET most effectively. The OPA derivatives of the native fluorescent bioamines emitted OPA fluorescence (445 nm) through an intermolecular FRET process when they were excited at the excitation maximum wavelengths of the native fluorescent bioamines (280 nm). The generation of FRET was confirmed through comparison with the analysis of a nonfluorescent amine (isoleucine) performed using LC and a three-dimensional fluorescence detection system. We were able to separate the OPA derivatives of the indoleamines and catecholamines when performing LC on an ODS column. The detection limits (signal-to-noise ratio, 3) for the indoleamines and catecholamines, at a 20-muL injection volume, were 17-120 and 28-200 fmol, respectively. The sensitivity of the intramolecular FRET-forming derivatization method is higher than those of systems that take advantage of both native fluorescence detection (i.e., without derivatization) and the conventional detection of OPA derivatives. Furthermore, this method provides enough selectivity and sensitivity for the determination of the indoleamines present in the urine of healthy humans. |
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Authors:
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Makoto Yoshitake; Hitoshi Nohta; Hideyuki Yoshida; Takashi Yoshitake; Kenichiro Todoroki; Masatoshi Yamaguchi |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Analytical chemistry Volume: 78 ISSN: 0003-2700 ISO Abbreviation: Anal. Chem. Publication Date: 2006 Feb |
Date Detail:
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Created Date: 2006-02-01 Completed Date: 2007-04-11 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0370536 Medline TA: Anal Chem Country: United States |
Other Details:
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Languages: eng Pagination: 920-7 Citation Subset: IM |
Affiliation:
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Faculty of Pharmaceutical Sciences, Fukuoka University, Nanakuma, Johnan, Japan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Biogenic Amines
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analysis* Chromatography, Liquid / methods Fluorescence Fluorescence Resonance Energy Transfer / methods* Humans Molecular Structure Sensitivity and Specificity |
| Chemical | |
Reg. No./Substance:
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0/Biogenic Amines |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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