| Selective determination of gamma-aminobutyric acid, glutamate and alanine by mixed micellar electrokinetic chromatography and fluorescence detection. | |
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MedLine Citation:
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PMID: 16221476 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A mixed micellar electrokinetic chromatography method with fluorescence detection was developed to simultaneously monitor gamma-aminobutyric acid (GABA), glutamate (Glu) and alanine (Ala) in biological samples. Amino acids were derivatized with naphthalene-2,3-dicarboxaldehyde (NDA). The separation of three NDA-labeled isomers (GABA, alpha-ABA, beta-ABA) was studied in detail with different micelles solutions such as sodium dodecyl sulfate (SDS), beta-cyclodextrin (beta-CD) and sodium cholate (SC). Simultaneous resolution of GABA, Glu and Ala from 21 amino acids was achieved within 5 min using 20 mM phosphate buffer at pH 8.7 containing 24 mM SC and 26 mM SDS. The detection limits were 4.0 x 10(-8), 1.1 x 10(-8) and 1.3 x 10(-8) M, for GABA, Glu and Ala, respectively, with S/N = 2. The method was applied to monitor the changes of amount of GABA, Glu and Ala in tobacco leaf in response to cold and dark stress. |
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Authors:
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Li-Yao Zhang; Meng-Xiang Sun |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2005-10-10 |
Journal Detail:
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Title: Journal of chromatography. A Volume: 1095 ISSN: 0021-9673 ISO Abbreviation: J Chromatogr A Publication Date: 2005 Nov |
Date Detail:
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Created Date: 2005-11-08 Completed Date: 2006-01-18 Revised Date: 2009-01-15 |
Medline Journal Info:
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Nlm Unique ID: 9318488 Medline TA: J Chromatogr A Country: Netherlands |
Other Details:
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Languages: eng Pagination: 185-8 Citation Subset: IM |
Affiliation:
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Key Laboratory of MOE for Plant Development Biology, College of Life Sciences, Wuhan University, China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Alanine
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analysis*,
chemistry Chromatography, Micellar Electrokinetic Capillary Cold Temperature Darkness Glutamic Acid / analysis*, chemistry Naphthalenes / chemistry Plant Leaves / chemistry Reproducibility of Results Tobacco / chemistry gamma-Aminobutyric Acid / analysis*, chemistry |
| Chemical | |
Reg. No./Substance:
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0/Naphthalenes; 56-12-2/gamma-Aminobutyric Acid; 56-41-7/Alanine; 56-86-0/Glutamic Acid; 7149-49-7/2,3-naphthalenedicarboxaldehyde |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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