Document Detail


Selective determination of catechin, epicatechin and ascorbic acid in human urine using chiral capillary electrophoresis.
MedLine Citation:
PMID:  18546394     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Chiral CE was successfully applied to the separation and quantification of catechin, epicatechin and ascorbic acid in some commercial drinks and human urine. Analysis involved the separation of analytes in less than 5.0 min at 240 nm with an untreated fused-silica capillary under hydrodynamic injection mode. The running buffer consisted of 50 mM borate buffer with 3 mM beta-CD at pH 8.35. Detection limits for catechin, epicatechin and ascorbic acid were 0.028, 0.011 and 0.004 microg/mL, respectively. Linearity was investigated by selecting the ranges of calibration according to the amount of analytes in urine giving correlation coefficient percent (% r(2)) ranging between 99.4 and 99.6 at 99% confidence level. The maximum urinary excretion of catechin and epicatechin were noted at 2.0 and 4.0 h of the administrated dose. Unchanged catechin, epicatechin and ascorbic acid amounted to about 1.500, 8.696 and 0.003% of the administered dose in the 48.0 h urine collection. The proposed method achieved 99.2% completeness (n = 20) in urine media.
Authors:
Deia Abd el-Hady; Roberto Gotti; Nagwa Abo el-Maali
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Publication Detail:
Type:  Journal Article; Validation Studies    
Journal Detail:
Title:  Journal of separation science     Volume:  31     ISSN:  1615-9314     ISO Abbreviation:  J Sep Sci     Publication Date:  2008 Jul 
Date Detail:
Created Date:  2008-07-17     Completed Date:  2008-10-09     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101088554     Medline TA:  J Sep Sci     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  2252-9     Citation Subset:  IM    
Affiliation:
Department of Chemistry, Faculty of Science, Assiut University, Assiut, Egypt. deiaabdelhady@yahoo.com
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MeSH Terms
Descriptor/Qualifier:
Antioxidants / analysis,  chemistry
Ascorbic Acid / urine*
Catechin / chemistry,  urine*
Coffee / chemistry
Electrophoresis, Capillary / methods*,  statistics & numerical data
Humans
Hydrogen-Ion Concentration
Sensitivity and Specificity
Stereoisomerism
Tea / chemistry
Chemical
Reg. No./Substance:
0/Antioxidants; 0/Coffee; 0/Tea; 154-23-4/Catechin; 50-81-7/Ascorbic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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