Selective determination of catechin, epicatechin and ascorbic acid in human urine using chiral capillary electrophoresis. | |
MedLine Citation:
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PMID: 18546394 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Chiral CE was successfully applied to the separation and quantification of catechin, epicatechin and ascorbic acid in some commercial drinks and human urine. Analysis involved the separation of analytes in less than 5.0 min at 240 nm with an untreated fused-silica capillary under hydrodynamic injection mode. The running buffer consisted of 50 mM borate buffer with 3 mM beta-CD at pH 8.35. Detection limits for catechin, epicatechin and ascorbic acid were 0.028, 0.011 and 0.004 microg/mL, respectively. Linearity was investigated by selecting the ranges of calibration according to the amount of analytes in urine giving correlation coefficient percent (% r(2)) ranging between 99.4 and 99.6 at 99% confidence level. The maximum urinary excretion of catechin and epicatechin were noted at 2.0 and 4.0 h of the administrated dose. Unchanged catechin, epicatechin and ascorbic acid amounted to about 1.500, 8.696 and 0.003% of the administered dose in the 48.0 h urine collection. The proposed method achieved 99.2% completeness (n = 20) in urine media. |
Authors:
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Deia Abd el-Hady; Roberto Gotti; Nagwa Abo el-Maali |
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Publication Detail:
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Type: Journal Article; Validation Studies |
Journal Detail:
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Title: Journal of separation science Volume: 31 ISSN: 1615-9314 ISO Abbreviation: J Sep Sci Publication Date: 2008 Jul |
Date Detail:
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Created Date: 2008-07-17 Completed Date: 2008-10-09 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101088554 Medline TA: J Sep Sci Country: Germany |
Other Details:
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Languages: eng Pagination: 2252-9 Citation Subset: IM |
Affiliation:
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Department of Chemistry, Faculty of Science, Assiut University, Assiut, Egypt. deiaabdelhady@yahoo.com |
Export Citation:
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MeSH Terms | |
Descriptor/Qualifier:
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Antioxidants
/
analysis,
chemistry Ascorbic Acid / urine* Catechin / chemistry, urine* Coffee / chemistry Electrophoresis, Capillary / methods*, statistics & numerical data Humans Hydrogen-Ion Concentration Sensitivity and Specificity Stereoisomerism Tea / chemistry |
Chemical | |
Reg. No./Substance:
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0/Antioxidants; 0/Coffee; 0/Tea; 154-23-4/Catechin; 50-81-7/Ascorbic Acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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