Document Detail

Selective detection of luminescence from semiconductor quantum dots by nanosecond time-gated imaging with a colour-masked CCD detector.
MedLine Citation:
PMID:  18445145     Owner:  NLM     Status:  PubMed-not-MEDLINE    
Quantum dots are of considerable interest as highly detectable labels with broad absorption, narrow spectral emission and good quantum yields. The luminescence emission has a longer decay time than that of the most common fluorophores, leading to facile rejection of much background emission (such as autofluorescence from biological samples) by means of gated detection. Here, it is shown that a new technique, true-colour nanosecond time-gated luminescence imaging, can be used for selective detection of quantum dot luminescence and should prove valuable for multiplexed detection on the basis of both spectral emission profile and luminescence decay time.
A C Mitchell; S Dad; C G Morgan
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of microscopy     Volume:  230     ISSN:  1365-2818     ISO Abbreviation:  J Microsc     Publication Date:  2008 May 
Date Detail:
Created Date:  2008-04-30     Completed Date:  2008-06-26     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0204522     Medline TA:  J Microsc     Country:  England    
Other Details:
Languages:  eng     Pagination:  172-6     Citation Subset:  -    
Biomedical Sciences Research Institute, School of Environmental and Life Sciences, University of Salford, Salford, United Kingdom.
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Grant Support
//Biotechnology and Biological Sciences Research Council

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