Document Detail


Selective detection of dopamine using a combined permselective film of electropolymerized (poly-tyramine and poly-pyrrole-1-propionic acid) on a boron-doped diamond electrode.
MedLine Citation:
PMID:  19238289     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
An effective and robust electrochemical approach has been developed for selective detection of dopamine in the presence of 3,4-dihydroxyphenylalanine (l-DOPA), ascorbic acid, uric acid and other dopamine metabolites. A 'layer-by-layer' film of tyramine and pyrrole-1-propionic acid (PPA) was formed by subsequent electropolymerization on a boron-doped diamond (BDD) electrode with an overall thickness of approximately 33 nm as estimated by AFM. The formation of the electropolymerized homogeneous film was also confirmed by SEM and Raman spectroscopy. The modified BDD electrode exhibited rapid response to dopamine within 6 s and a detection limit of 50 nM with excellent reproducibility. The stable electropolymerized film was capable of excluding electroactive interference from 20 microM l-DOPA, 20 microM 3,4-dihydroxyphenylacetic acid (DOPAC), and ascorbic and uric acids at normal physiological conditions (100 microM each). The modified electrode could be used for several repeated analyses of dopamine at 5 microM, without noticeable surface fouling. A plausible mechanism for permselectivity was suggested and supported by pertinent experimental data.
Authors:
Fengjun Shang; Yali Liu; Sabahudin Hrapovic; Jeremy D Glennon; John H T Luong
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Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-12-05
Journal Detail:
Title:  The Analyst     Volume:  134     ISSN:  1364-5528     ISO Abbreviation:  Analyst     Publication Date:  2009 Mar 
Date Detail:
Created Date:  2009-02-24     Completed Date:  2010-01-28     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372652     Medline TA:  Analyst     Country:  England    
Other Details:
Languages:  eng     Pagination:  519-27     Citation Subset:  IM    
Affiliation:
Department of Chemistry and Analytical and Biological Chemistry Research Facility (ABCRF), University College Cork, Cork, Ireland.
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MeSH Terms
Descriptor/Qualifier:
Animals
Biosensing Techniques / instrumentation,  methods
Boron / chemistry
Dopamine / analysis*
Electrochemical Techniques / instrumentation*,  methods
Electrodes
Pyrroles / chemistry
Tyramine / chemistry
Chemical
Reg. No./Substance:
0/Pyrroles; 51-67-2/Tyramine; 7440-42-8/Boron

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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