Document Detail


Selective depletion of the Type I, Type II, and Type III isozymes of hexokinase in mammalian cells using small interfering RNAs.
MedLine Citation:
PMID:  15184049     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Constructs based on the pSUPER vector [Science 296 (2002) 550] and encoding small interfering RNAs specific for the Type I, Type II, or Type III isozymes of mammalian (rat) hexokinase were prepared. Transfection of Chinese hamster ovary and HeLa cells with these vectors resulted in selective depletion of the respective isozymes. A Zeocin marker was incorporated into the modified pSUPER vector, permitting isolation of stably transfected cell lines selectively depleted of the respective isozyme.
Authors:
Dexin Sui; John E Wilson
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  319     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  2004 Jul 
Date Detail:
Created Date:  2004-06-08     Completed Date:  2004-07-30     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  768-73     Citation Subset:  IM    
Affiliation:
Department of Biochemistry and Molecular Biology, Michigan State University, East Lansing, MI 48824, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
CHO Cells
Cell Line
Cricetinae
Genetic Vectors / genetics,  metabolism
Hexokinase / genetics,  metabolism*
Humans
Isoenzymes / genetics,  metabolism*
RNA, Small Interfering / genetics,  metabolism*
Rats
Transfection
Grant Support
ID/Acronym/Agency:
NS 09910/NS/NINDS NIH HHS
Chemical
Reg. No./Substance:
0/Isoenzymes; 0/RNA, Small Interfering; EC 2.7.1.1/Hexokinase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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