| Selective culture medium enhances survival of neuroblasts from postnatal rodent brain. | |
| | |
MedLine Citation:
|
PMID: 3756538 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
One of the major limitations to the study of the development of the nervous system in aneuploid mammalian embryos is that the aneuploid condition is usually lethal in utero. Liveborn aneuploid individuals often succumb rapidly because they have a constellation of malformations incompatible with postnatal survival. We have developed a selective tissue culture medium (D-val) which can be used with fetal calf serum or in a serum-free defined composition and which permits neuroblasts and glioblasts present in postnatal rodent (rat, mouse) brain to proliferate and differentiate in vitro. These cells contain D-amino acid oxidase, but fibroblasts do not. In this medium, fibroblasts cannot grow and are eliminated from the cultures without the use of deleterious compounds such as antimitotic or chemotherapeutic agents. With this medium, cultures containing neuroblasts can be established from normal rat and mouse brain as late as postnatal days 11-20. Cultures with significant numbers of proliferating cells can also be established from perinatal aneuploid mouse embryos, 'rescuing' the cells for further study. |
| | |
Authors:
|
S F Gilbert; M L Oster-Granite; W Kirby; D Durie; D Lyons; E Greene |
Related Documents
:
|
16660748 - Freeze preservation of somatic embryos and clonal plantlets of carrot (daucus carota l). 17934308 - Soluble hla-g and embryo implantation: frequently asked questions. 16664558 - Identification and isolation of single cells that produce somatic embryos at a high fre... 16672718 - Cloned transgenic swine via in vitro production and cryopreservation. 6982298 - An efficient method for purification of human t-cell growth factor. 3911818 - Cultivation of fungi in synthetic and semi-synthetic liquid medium. ii. immunochemical ... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
|
Title: Brain research bulletin Volume: 16 ISSN: 0361-9230 ISO Abbreviation: Brain Res. Bull. Publication Date: 1986 Jun |
Date Detail:
|
Created Date: 1986-11-18 Completed Date: 1986-11-18 Revised Date: 2007-11-14 |
Medline Journal Info:
|
Nlm Unique ID: 7605818 Medline TA: Brain Res Bull Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 853-60 Citation Subset: IM |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Animals, Newborn / physiology Brain / cytology* Cell Survival / drug effects Culture Media / pharmacology* Culture Techniques / methods Mice Mice, Inbred BALB C Mice, Neurologic Mutants Neurons / cytology*, drug effects Rats Rats, Inbred Strains Stem Cells / cytology*, drug effects Trisomy Valine / pharmacology* |
| Grant Support | |
ID/Acronym/Agency:
|
HD-15032/HD/NICHD NIH HHS; HD-19932/HD/NICHD NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Culture Media; 7004-03-7/Valine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Histogenesis of the retina in murine trisomy 19.
Next Document: A new microcirculation chamber for inexpensive long-term investigations of nervous tissue in vitro.