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Selective co-expression of synaptic proteins, α-synuclein, cysteine string protein-α, synaptophysin, synaptotagmin-1, and synaptobrevin-2 in vesicular acetylcholine transporter-immunoreactive axons in the guinea-pig ileum.
MedLine Citation:
PMID:  23296877     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Parkinson's disease is a neurodegenerative disorder characterized by Lewy bodies and neurites composed mainly of the presynaptic protein, α-synuclein. Frequently, Lewy bodies and neurites are identified in the gut of Parkinson's disease patients and may underlie associated gastrointestinal dysfunctions. We recently reported selective expression of α-synuclein in the axons of cholinergic neurons in the guinea-pig and human distal gut; however, it is not clear if α-synuclein expression varies along the gut, nor how closely expression is associated with other synaptic proteins. We used multiple-labeling immunohistochemistry to quantify which neurons in the guinea-pig ileum expressed α-synuclein, cysteine string protein-α (CSPα), synaptophysin, synaptotagmin-1, or synaptobrevin-2 in their axons. Of the 10 neurochemically-defined axonal populations, a significantly greater proportion of vesicular acetylcholine transporter-immunoreactive (VAChT-IR) varicosities (80 ± 1.7%, n=4, P < 0.001) contained α-synuclein-immunoreactivity, and a significantly greater proportion of α-synuclein-IR axons also contained VAChT-immunoreactivity (78 ± 1.3%, n=4), compared to any of the other 9 populations (P < 0.001 in all cases). Of synaptophysin-, synaptotagmin-1-, synaptobrevin-2-, and CSPα-IR varicosities, 98 ± 0.7%, 96 ± 0.7%, 88 ± 1.6% and 85 ± 2.9% (n=4) contained α-synuclein-immunoreactivity, respectively. Of α-synuclein-IR varicosities, 96 ± 0.9%, 99 ± 0.6%, 83 ± 1.9% and 87 ± 2.3% (n=4) contained synaptophysin-, synaptotagmin-1-, synaptobrevin-2-, and CSPα-immunoreactivity, respectively. We report a close association between the expression of α-synuclein and other synaptic proteins in cholinergic axons in the guinea-pig ileum. Selective expression of α-synuclein may relate to the neurotransmitter system utilized and predispose cholinergic enteric neurons to neurodegeneration in Parkinson's disease. J. Comp. Neurol., 2013. © 2013 Wiley Periodicals, Inc.
Authors:
Dale F Sharrad; Wei-Ping Gai; Simon J H Brookes
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-8
Journal Detail:
Title:  The Journal of comparative neurology     Volume:  -     ISSN:  1096-9861     ISO Abbreviation:  J. Comp. Neurol.     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-8     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0406041     Medline TA:  J Comp Neurol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2013 Wiley Periodicals, Inc.
Affiliation:
Department of Human Physiology and Centre for Neuroscience, Flinders Medical Science and Technology, School of Medicine, Flinders University, Bedford Park, South Australia, Australia.
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