Document Detail

Selective cell transplantation using bioabsorbable artificial polymers as matrices.
MedLine Citation:
PMID:  2895175     Owner:  NLM     Status:  MEDLINE    
To date, selective cell transplantation has involved injecting cell suspensions into tissues or the vascular system. This study describes attaching cell preparations to bioerodable artificial polymers in cell culture and then implanting this polymer-cell scaffold into animals. Using standard techniques of cell harvest, single cells and clusters of fetal and adult rat and mouse hepatocytes, pancreatic islet cells, and small intestinal cells have been seeded onto biodegradable polymers of polyglactin 910, polyanhydrides, and polyorthoester. Sixty-five fetuses and 14 adult animals served as donors. One hundred fifteen polymer scaffolds were implanted into 70 recipient animals: 66 seeded with hepatocytes; 23 with intestinal cells and clusters; and 26 with pancreatic islet preparations. The cells remained viable in culture, and in the case of fetal intestine and fetal hepatocytes, appeared to proliferate while on the polymer. After four days in culture, the cell-polymer scaffolds were implanted into host animals, either in the omentum, the interscapular fat pad, or the mesentery. In three cases of fetal intestinal implantation coupled with partial hepatectomy, successful engraftment occurred in the omentum, one forming a visible 6.0 mm cyst. Three cases of hepatocyte implantation, one using adult cells and two using fetal cells, have also engrafted, showing viability of hepatocytes, mitotic figures, and vascularization of the cell mass. To date, no pancreatic islets have survived implantation. This method of cell transplantation, which we have termed "chimeric neomorphogenesis," is an alternative to current methods and requires further study.
J P Vacanti; M A Morse; W M Saltzman; A J Domb; A Perez-Atayde; R Langer
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of pediatric surgery     Volume:  23     ISSN:  0022-3468     ISO Abbreviation:  J. Pediatr. Surg.     Publication Date:  1988 Jan 
Date Detail:
Created Date:  1988-05-06     Completed Date:  1988-05-06     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0052631     Medline TA:  J Pediatr Surg     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  3-9     Citation Subset:  IM    
Department of Surgery, Children's Hospital, Boston, MA 02115.
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MeSH Terms
Biocompatible Materials*
Cell Survival
Cell Transplantation*
Cells, Cultured
Decanoic Acids*
Islets of Langerhans
Mice, Inbred C57BL
Polyglactin 910*
Rats, Inbred Strains
Grant Support
6M 26698//PHS HHS
Reg. No./Substance:
0/Biocompatible Materials; 0/Decanoic Acids; 0/Polyesters; 0/Polymers; 34346-01-5/Polyglactin 910; 88849-65-4/1,4-cyclohexanedimethanol-3,9-diethylidene-2,4,8,10-tetraoxaspiro(5.5)undecane-1,6-hexanediol polymer; 90409-78-2/decanedioic acid-4,4'-(1,3-propanediylbis(oxy))bis(benzoic acid) copolymer

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