Document Detail


Selective binding of heat- and antigen-aggregated IgG to Blue-Sepharose. Radioimmunoassay of circulating immune complexes.
MedLine Citation:
PMID:  6655240     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In an attempt to find a direct method for the determination of circulating immune complexes in pathological sera we studied the ability of Cibacron Blue 3GA covalently bound to Sepharose 4B to bind heat- or antigen-aggregated human IgG. Blue-Sepharose was found to bind significant amounts of aggregated IgG (about 1.5 mg/ml packed gel) but very low amounts of monomeric IgG (about 0.1 mg/ml gel). The ability of Blue-Sepharose to bind immune complexes was tested on human pathological sera containing variable amounts of circulating immune complexes. Using both a turbidimetric measurement and Barkas' method we found that Blue-Sepharose is able to remove a very high percent of circulating immune complexes (greater than 80%) from sera. Considering the above observations a new method is described for the direct determination of circulating immune complexes using Blue-Sepharose as serum adsorbent and either 125I-labeled protein A or 125I-labeled rabbit IgG anti-human IgG to demonstrate the binding of immune complexes.
Authors:
M A Dobre; G Moţa
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of immunological methods     Volume:  65     ISSN:  0022-1759     ISO Abbreviation:  J. Immunol. Methods     Publication Date:  1983 Dec 
Date Detail:
Created Date:  1984-02-14     Completed Date:  1984-02-14     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  1305440     Medline TA:  J Immunol Methods     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  207-15     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Antigen-Antibody Complex / analysis*,  metabolism
Binding Sites, Antibody*
Binding, Competitive
Blood Proteins / analysis
Hot Temperature
Humans
Immunoglobulin G / metabolism*
Rabbits
Radioimmunoassay / methods*
Sepharose / analogs & derivatives*,  diagnostic use
Staphylococcal Protein A / diagnostic use
Chemical
Reg. No./Substance:
0/Antigen-Antibody Complex; 0/Binding Sites, Antibody; 0/Blood Proteins; 0/Immunoglobulin G; 0/Staphylococcal Protein A; 66456-82-4/Blue-Sepharose; 9012-36-6/Sepharose

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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