| Selective autophagy degrades DICER and AGO2 and regulates miRNA activity. | |
| | |
MedLine Citation:
|
PMID: 23143396 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
MicroRNAs (miRNAs) form a class of short RNAs (∼ 21 nucleotides) that post-transcriptionally regulate partially complementary messenger RNAs. Each miRNA may target tens to hundreds of transcripts to control key biological processes. Although the biochemical reactions underpinning miRNA biogenesis and activity are relatively well defined and the importance of their homeostasis is increasingly evident, the processes underlying regulation of the miRNA pathway in vivo are still largely elusive. Autophagy, a degradative process in which cytoplasmic material is targeted into double-membrane vacuoles, is recognized to critically contribute to cellular homeostasis. Here, we show that the miRNA-processing enzyme, DICER (also known as DICER1), and the main miRNA effector, AGO2 (also known as eukaryotic translation initiation factor 2C, 2 (EIF2C2)), are targeted for degradation as miRNA-free entities by the selective autophagy receptor NDP52 (also known as calcium binding and coiled-coil domain 2 (CALCOCO2)). Autophagy establishes a checkpoint required for continued loading of miRNA into AGO2; accordingly, NDP52 and autophagy are required for homeostasis and activity of the tested miRNAs. Autophagy also engages post-transcriptional regulation of the DICER mRNA, underscoring the importance of fine-tuned regulation of the miRNA pathway. These findings have implications for human diseases linked to misregulated autophagy, DICER- and miRNA-levels, including cancer. |
| | |
Authors:
|
Derrick Gibbings; Serge Mostowy; Florence Jay; Yannick Schwab; Pascale Cossart; Olivier Voinnet |
Related Documents
:
|
23571096 - Somewhat in control-the role of transcription in regulating microbial metabolic fluxes. 23611506 - Comparing functional metabolic effects of marginal and sufficient selenium supply in sh... 22507326 - Aberrant beta-catenin and lef1 expression may predict the clinical outcome for patients... 9464256 - Tex261, a novel gene presumably related but distinct from steroidogenic acute regulator... 15835916 - Kruppel-like factor 2 transcriptional regulation involves heterogeneous nuclear ribonuc... 18768156 - Cell cycle activation in striatal neurons from huntington's disease patients and rats t... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Nature cell biology Volume: 14 ISSN: 1476-4679 ISO Abbreviation: Nat. Cell Biol. Publication Date: 2012 Dec |
Date Detail:
|
Created Date: 2013-01-17 Completed Date: 2013-01-28 Revised Date: 2013-02-06 |
Medline Journal Info:
|
Nlm Unique ID: 100890575 Medline TA: Nat Cell Biol Country: England |
Other Details:
|
Languages: eng Pagination: 1314-21 Citation Subset: IM |
Affiliation:
|
Swiss Federal Institute of Technology (ETH-Z), Department of Biology, Zürich 8092, Switzerland. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Argonaute Proteins
/
genetics,
metabolism* Autophagy / genetics, physiology* Cell Line Cell Line, Tumor HeLa Cells Humans Immunoprecipitation MicroRNAs / metabolism* Microscopy, Confocal Microscopy, Electron Nuclear Proteins / genetics, metabolism Reverse Transcriptase Polymerase Chain Reaction Ribonuclease III / genetics, metabolism* |
| Grant Support | |
ID/Acronym/Agency:
|
//Wellcome Trust |
| Chemical | |
Reg. No./Substance:
|
0/Argonaute Proteins; 0/EIF2C2 protein, human; 0/MicroRNAs; 0/Nuclear Proteins; 0/nuclear dot protein 52, human; EC 3.1.26.3/Ribonuclease III |
| Comments/Corrections | |
Comment In:
|
Nat Rev Mol Cell Biol. 2013 Jan;14(1):4-5
[PMID:
23212473
]
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: YAP mediates crosstalk between the Hippo and PI(3)K–TOR pathways by suppressing PTEN via miR-29.
Next Document: Coordinated oscillations in cortical actin and Ca2+ correlate with cycles of vesicle secretion.