Document Detail

Selective amino acids changes in the medial and lateral preoptic area in the formalin test in rats.
MedLine Citation:
PMID:  14980389     Owner:  NLM     Status:  MEDLINE    
A combination of microdialysis in freely moving rats and capillary zone electrophoresis coupled to laser induced fluorescence detection was used to measure extracellular concentrations of amino acid neurotransmitters in different hypothalamic areas during noxious stimulation. Arginine, glutamate and aspartate were monitored every 30 s before and after a s.c. injection of formalin (5%, 50 microl) or saline (0.9%) in the right hind paw. In the medial and lateral preoptic area, calcium and nerve impulse dependent increases of arginine, glutamate and aspartate were observed during the first 2 min after formalin injection. However, amino acid changes were not detected in the lateral hypothalamus or in the ventromedial nucleus when compared with pre-injection levels or with the levels from animals injected with saline in the hind paw. Flinching behavior was also scored during the first 10 min following the formalin or saline injection. Flinching frequency was maximum at minute 2 after formalin injection, whereas saline injection did not elicited any flinching behavior. These results show that nociceptive stimulation induces rapid and differential amino acids changes in discrete areas of the hypothalamus that can be associated with pain-related behavior.
E Silva; L Hernandez; B Quiñonez; L E Gonzalez; C Colasante
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Neuroscience     Volume:  124     ISSN:  0306-4522     ISO Abbreviation:  Neuroscience     Publication Date:  2004  
Date Detail:
Created Date:  2004-02-24     Completed Date:  2004-06-01     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7605074     Medline TA:  Neuroscience     Country:  United States    
Other Details:
Languages:  eng     Pagination:  395-404     Citation Subset:  IM    
Laboratory of Behavioral Physiology, School of Medicine, Universidad de Los Andes, Merida, Venezuela.
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MeSH Terms
Amino Acids / metabolism*
Analysis of Variance
Behavior, Animal
Brain Chemistry
Calcium / metabolism
Electrophoresis, Capillary / methods
Hypothalamus / anatomy & histology,  drug effects,  metabolism
Microdialysis / methods
Pain / chemically induced,  metabolism*
Pain Measurement
Preoptic Area / anatomy & histology,  metabolism*
Rats, Wistar
Time Factors
Triazines / pharmacology
Reg. No./Substance:
0/Amino Acids; 0/Triazines; 2451-62-9/1,3,5-triglycidyl-s-triazinetrione; 50-00-0/Formaldehyde; 7440-70-2/Calcium

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