| Selective adsorption of hydrocarbon-oxidizing Rhodococcus cells in a column with hydrophobized poly(acrylamide) cryogel. | |
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MedLine Citation:
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PMID: 19682505 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A method for selective adsorption of Rhodococcus cells in the column with hydrophobized poly(acrylamide) cryogel (cryoPAAG) was developed that allowed rhodococci separation from mixed bacterial populations and their effective concentration within a sponge-like gel matrix. Hydrophobization of cryoPAAG using the n-dodecane graft (C12) was performed to enhance the adhesion of Rhodococcus cells to the cryogel; this was suggested by our finding that alkanotrophic rhodococci possess high adhesive activity (91-98%) towards n-alkanes, whereas other Gram-positive and Gram-negative bacteria tested did not adhere strongly to hydrocarbons. The selective index of the hydrophobic C12-cryoPAAG column for Rhodococcus cells was 72% that ensured their separation from complex bacterial cultures. Respirometry results using the Columbus Micro-Oxymax respirometer showed that the maximal respiratory activity of C12-cryoPAAG-immobilized Rhodococcus cells incubated with petroleum hydrocarbons was 1.6-1.8 times higher than that of freely suspended cells, and this correlated with the largest immobilized cell number. Moreover, high respiration rates were maintained over 3 weeks of incubation, indicating a considerable functional stability of the cryoPAAG-immobilized biocatalyst developed. |
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Authors:
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Maria S Kuyukina; Ekaterina V Rubtsova; Irena B Ivshina; Roman V Ivanov; Vladimir I Lozinsky |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-08-12 |
Journal Detail:
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Title: Journal of microbiological methods Volume: 79 ISSN: 1872-8359 ISO Abbreviation: J. Microbiol. Methods Publication Date: 2009 Oct |
Date Detail:
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Created Date: 2009-09-28 Completed Date: 2009-11-17 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8306883 Medline TA: J Microbiol Methods Country: Netherlands |
Other Details:
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Languages: eng Pagination: 76-81 Citation Subset: IM |
Affiliation:
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Institute of Ecology and Genetics of Microorganisms, Russian Academy of Sciences, Perm, Russia. kuyukina@iegm.ru |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Acrylic Resins
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chemistry* Adsorption Bacteriological Techniques / methods Hydrocarbons / metabolism* Hydrogels / chemistry* Oxidation-Reduction Rhodococcus / isolation & purification*, metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Acrylic Resins; 0/Hydrocarbons; 0/Hydrogels; 0/cryogel; 9003-05-8/polyacrylamide |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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