Document Detail


Selective adsorption of hydrocarbon-oxidizing Rhodococcus cells in a column with hydrophobized poly(acrylamide) cryogel.
MedLine Citation:
PMID:  19682505     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A method for selective adsorption of Rhodococcus cells in the column with hydrophobized poly(acrylamide) cryogel (cryoPAAG) was developed that allowed rhodococci separation from mixed bacterial populations and their effective concentration within a sponge-like gel matrix. Hydrophobization of cryoPAAG using the n-dodecane graft (C12) was performed to enhance the adhesion of Rhodococcus cells to the cryogel; this was suggested by our finding that alkanotrophic rhodococci possess high adhesive activity (91-98%) towards n-alkanes, whereas other Gram-positive and Gram-negative bacteria tested did not adhere strongly to hydrocarbons. The selective index of the hydrophobic C12-cryoPAAG column for Rhodococcus cells was 72% that ensured their separation from complex bacterial cultures. Respirometry results using the Columbus Micro-Oxymax respirometer showed that the maximal respiratory activity of C12-cryoPAAG-immobilized Rhodococcus cells incubated with petroleum hydrocarbons was 1.6-1.8 times higher than that of freely suspended cells, and this correlated with the largest immobilized cell number. Moreover, high respiration rates were maintained over 3 weeks of incubation, indicating a considerable functional stability of the cryoPAAG-immobilized biocatalyst developed.
Authors:
Maria S Kuyukina; Ekaterina V Rubtsova; Irena B Ivshina; Roman V Ivanov; Vladimir I Lozinsky
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-08-12
Journal Detail:
Title:  Journal of microbiological methods     Volume:  79     ISSN:  1872-8359     ISO Abbreviation:  J. Microbiol. Methods     Publication Date:  2009 Oct 
Date Detail:
Created Date:  2009-09-28     Completed Date:  2009-11-17     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8306883     Medline TA:  J Microbiol Methods     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  76-81     Citation Subset:  IM    
Affiliation:
Institute of Ecology and Genetics of Microorganisms, Russian Academy of Sciences, Perm, Russia. kuyukina@iegm.ru
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MeSH Terms
Descriptor/Qualifier:
Acrylic Resins / chemistry*
Adsorption
Bacteriological Techniques / methods
Hydrocarbons / metabolism*
Hydrogels / chemistry*
Oxidation-Reduction
Rhodococcus / isolation & purification*,  metabolism*
Chemical
Reg. No./Substance:
0/Acrylic Resins; 0/Hydrocarbons; 0/Hydrogels; 0/cryogel; 9003-05-8/polyacrylamide

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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