| Selective action of human sera differing in fatty acids and cholesterol content on in vitro gene expression. | |
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MedLine Citation:
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PMID: 22021027 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Serum constituents might directly affect metabolic diseases pathogenesis and are commonly used as diagnostic tool. The aim of this study was to investigate the human serum effect on in vitro gene expression, related to nutrients action and involved in lipid metabolism. In detail, forty human sera were firstly analyzed in fatty acids profile by gas-chromatography. Then samples were tested through direct addition within culture medium on Hep G2 human hepatoma cells, comparing samples from hypercholesterolemic (average 273 mg/dl) vs normocholesterolemic male subjects (average 155 mg/dl), since this condition is a relevant disease risk factor and is typically consequent to nutritional style. Hypercholesterolemic sera produced a 0.4-fold reduction of Sterol Regulatory Element Binding Protein 1c (SREBP-1c) mRNA (p < 0.05) and a 1.5-fold increase of UDP-Glucuronosyltransferase 1A1 (UGT1A1) mRNA (p < 0.01). Samples with higher concentrations of n-6 fatty acids produced a higher expression of UGT1A1 mRNA. Total fatty acids [docosahexaenoic, eicosopentanoic, arachidonic, linolenic and linoleic acid (DHA, EPA, AA, LNA and LA, respectively)] in each serum resulted roughly inverse with trend of SREBP-1c mRNA expression. Serum AA, LA and trans fatty acids were more abundant in hypercholesterolemic subjects (p < 0.01) while DHA as quota of detected fatty acids was significantly higher in normocholesterolemic subjects (p < 0.05). While it is not possible to indicate which component was responsible for the observed gene modulations, our data indicate that sera differing in lipid profiles, mainly associated with dietary behavior, differentially affect gene expression known to be involved in metabolic and nutritional related conditions. J. Cell. Biochem. © 2011 Wiley Periodicals, Inc. |
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Authors:
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Hylde Zirpoli; Mariella Caputo; Antonio Carraturo; Gaetano Torino; Alessia Fazio; Mohamed Attya; Luca Rastrelli; Mario Felice Tecce |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-10-20 |
Journal Detail:
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Title: Journal of cellular biochemistry Volume: - ISSN: 1097-4644 ISO Abbreviation: - Publication Date: 2011 Oct |
Date Detail:
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Created Date: 2011-10-24 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8205768 Medline TA: J Cell Biochem Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2011 Wiley Periodicals, Inc. |
Affiliation:
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Department of Pharmaceutical and Biomedical Sciences, University of Salerno, via Ponte Don Melillo, 84084 Fisciano, Salerno, Italy. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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