| Selective (R)-3-hydroxylation of FA by Stenotrophomonas maltophilia. | |
| | |
MedLine Citation:
|
PMID: 11942484 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Soil samples were screened for microorganisms selectively transforming FA. One of the isolated strains was identified as the bacterium Stenotrophomonas maltophilia by its phenotypic features and genotypic characterization by sequencing the ribosomal RNA gene. Using linoleic acid as substrate resulted in the formation of two major compounds. After liquid chromatographic isolation and separation, their structures were elucidated by HPLC-tandem MS, GC-MS, and NMR techniques to be 3-hydroxy-Z6-dodecenoic acid and 3-hydroxy-Z5,Z8-tetradecadienoic acid. In additional experiments, other FA, such as a-linolenic, oleic, palmitoleic, myristoleic, and cis-vaccenic acids, were converted to 3-hydroxylated metabolites of shorter chain lengths as well. Determination of the enantiomeric composition revealed highly enriched (R)-hydroxylation (88-98% enantiomeric excess). |
| | |
Authors:
|
Kerstin Weil; Patrick Gruber; Frank Heckel; Dag Harmsen; Peter Schreier |
Related Documents
:
|
11518224 - Dietary conjugated linoleic acid alters fatty acid composition of pig skeletal muscle a... 12030324 - Biosynthesis of new divinyl ether oxylipins in ranunculus plants. 6378594 - The degradation of monoiodotyrosyl insulin isomers by insulin protease. 18304714 - Effect of acute lindane and alcohol intoxication on serum concentration of enzymes and ... 6848664 - Effects of cdpcholine and cdpethanolamine on the alterations in rat brain lipid metabol... 6108604 - Effect of two types of beta-adrenergic blockade on gastric acid secretion during basal ... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Lipids Volume: 37 ISSN: 0024-4201 ISO Abbreviation: Lipids Publication Date: 2002 Mar |
Date Detail:
|
Created Date: 2002-04-10 Completed Date: 2002-10-10 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 0060450 Medline TA: Lipids Country: United States |
Other Details:
|
Languages: eng Pagination: 317-23 Citation Subset: IM |
Affiliation:
|
Lehrstuhl für Lebensmittelchemie, Universität Würzburg, Germany. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Chromatography, High Pressure Liquid Fatty Acids / metabolism* Genotype Hydroxylation Magnetic Resonance Spectroscopy Phenotype Spectrometry, Mass, Electrospray Ionization Stenotrophomonas maltophilia / genetics, metabolism* |
| Chemical | |
Reg. No./Substance:
|
0/Fatty Acids |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Changes in lipid molecular species and sterols of microsomal membranes during aging of potato (Solan...
Next Document: Saturated and unsaturated anteiso-C19 acids in the seed lipids from Hesperopeuce mertensiana (Pinace...