| Selective quantification by 2D HSQC0 spectroscopy of thiocoraline in an extract from a sponge-derived Verrucosispora sp. | |
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MedLine Citation:
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PMID: 21923106 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We recently developed a 2D 1H-13C HSQC0 approach to quantify individual chemicals in complex mixtures. The HSQC0 approach has been implemented in phase-cycled and gradient-selective versions. As in quantitative 1D NMR, the normalized integrated signal intensities in HSQC0 are proportional to the concentrations of individual chemicals in the mixture. We applied the HSQC0 approach to selectively quantify thiocoraline present at a level of 1% w/w in an extract from a Verrucosispora sp. isolated from the sponge Chondrilla caribensis f. caribensis. We expect that this approach can be used to quantify other natural products of interest in extracts without prior purification. |
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Authors:
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Kaifeng Hu; Thomas P Wyche; Tim S Bugni; John L Markley |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2011-09-16 |
Journal Detail:
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Title: Journal of natural products Volume: 74 ISSN: 1520-6025 ISO Abbreviation: J. Nat. Prod. Publication Date: 2011 Oct |
Date Detail:
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Created Date: 2011-10-28 Completed Date: 2012-01-09 Revised Date: 2013-02-19 |
Medline Journal Info:
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Nlm Unique ID: 7906882 Medline TA: J Nat Prod Country: United States |
Other Details:
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Languages: eng Pagination: 2295-8 Citation Subset: IM |
Affiliation:
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National Magnetic Resonance Facility at Madison, Biochemistry Department, University of Wisconsin-Madison, Madison, Wisconsin 53706, United States. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Depsipeptides / analysis*, chemistry Micromonosporaceae / chemistry* Molecular Structure Nuclear Magnetic Resonance, Biomolecular Porifera / microbiology* |
| Grant Support | |
ID/Acronym/Agency:
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1S10RR025062/RR/NCRR NIH HHS; P41 RR002301-25/RR/NCRR NIH HHS; P41 RR002301-25S1/RR/NCRR NIH HHS; P41RR02301/RR/NCRR NIH HHS; S10 RR025062-01/RR/NCRR NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Depsipeptides; 0/thiocoraline |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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