Document Detail


Selective quantification by 2D HSQC0 spectroscopy of thiocoraline in an extract from a sponge-derived Verrucosispora sp.
MedLine Citation:
PMID:  21923106     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We recently developed a 2D 1H-13C HSQC0 approach to quantify individual chemicals in complex mixtures. The HSQC0 approach has been implemented in phase-cycled and gradient-selective versions. As in quantitative 1D NMR, the normalized integrated signal intensities in HSQC0 are proportional to the concentrations of individual chemicals in the mixture. We applied the HSQC0 approach to selectively quantify thiocoraline present at a level of 1% w/w in an extract from a Verrucosispora sp. isolated from the sponge Chondrilla caribensis f. caribensis. We expect that this approach can be used to quantify other natural products of interest in extracts without prior purification.
Authors:
Kaifeng Hu; Thomas P Wyche; Tim S Bugni; John L Markley
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2011-09-16
Journal Detail:
Title:  Journal of natural products     Volume:  74     ISSN:  1520-6025     ISO Abbreviation:  J. Nat. Prod.     Publication Date:  2011 Oct 
Date Detail:
Created Date:  2011-10-28     Completed Date:  2012-01-09     Revised Date:  2014-09-22    
Medline Journal Info:
Nlm Unique ID:  7906882     Medline TA:  J Nat Prod     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2295-8     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Depsipeptides / analysis*,  chemistry
Micromonosporaceae / chemistry*
Molecular Structure
Nuclear Magnetic Resonance, Biomolecular
Porifera / microbiology*
Grant Support
ID/Acronym/Agency:
1S10RR025062/RR/NCRR NIH HHS; P41 RR002301/RR/NCRR NIH HHS; P41 RR002301-25/RR/NCRR NIH HHS; P41 RR002301-25S1/RR/NCRR NIH HHS; P41RR02301/RR/NCRR NIH HHS; R01 GM092009/GM/NIGMS NIH HHS; S10 RR025062/RR/NCRR NIH HHS; S10 RR025062-01/RR/NCRR NIH HHS
Chemical
Reg. No./Substance:
0/Depsipeptides; 0/thiocoraline
Comments/Corrections

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