| Selection of fecal enterococci exhibiting tcrB-mediated copper resistance in pigs fed diets supplemented with copper. | |
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MedLine Citation:
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PMID: 21705534 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Copper, as copper sulfate, is increasingly used as an alternative to in-feed antibiotics for growth promotion in weaned piglets. Acquired copper resistance, conferred by a plasmid-borne, transferable copper resistance (tcrB) gene, has been reported in Enterococcus faecium and E. faecalis. A longitudinal field study was undertaken to determine the relationship between copper supplementation and the prevalence of tcrB-positive enterococci in piglets. The study was done with weaned piglets, housed in 10 pens with 6 piglets per pen, fed diets supplemented with a normal (16.5 ppm; control) or an elevated (125 ppm) level of copper. Fecal samples were randomly collected from three piglets per pen on days 0, 14, 28, and 42 and plated on M-Enterococcus agar, and three enterococcal isolates were obtained from each sample. The overall prevalence of tcrB-positive enterococci was 21.1% (38/180) in piglets fed elevated copper and 2.8% (5/180) in the control. Among the 43 tcrB-positive isolates, 35 were E. faecium and 8 were E. faecalis. The mean MICs of copper for tcrB-negative and tcrB-positive enterococci were 6.2 and 22.2 mM, respectively. The restriction digestion of the genomic DNA of E. faecium or E. faecalis with S1 nuclease yielded a band of ∼194-kbp size to which both tcrB and the erm(B) gene probes hybridized. A conjugation assay demonstrated cotransfer of tcrB and erm(B) genes between E. faecium and E. faecalis strains. The higher prevalence of tcrB-positive enterococci in piglets fed elevated copper compared to that in piglets fed normal copper suggests that supplementation of copper in swine diets selected for resistance. |
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Authors:
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R G Amachawadi; N W Shelton; X Shi; J Vinasco; S S Dritz; M D Tokach; J L Nelssen; H M Scott; T G Nagaraja |
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Publication Detail:
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Type: Journal Article; Randomized Controlled Trial Date: 2011-06-24 |
Journal Detail:
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Title: Applied and environmental microbiology Volume: 77 ISSN: 1098-5336 ISO Abbreviation: Appl. Environ. Microbiol. Publication Date: 2011 Aug |
Date Detail:
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Created Date: 2011-08-05 Completed Date: 2011-12-15 Revised Date: 2012-02-01 |
Medline Journal Info:
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Nlm Unique ID: 7605801 Medline TA: Appl Environ Microbiol Country: United States |
Other Details:
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Languages: eng Pagination: 5597-603 Citation Subset: IM |
Affiliation:
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Department of Diagnostic Medicine/Pathobiology, Kansas State University, Manhattan, KS 66506-5606, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animal Feed Animals Bacterial Proteins / genetics, metabolism* Bacterial Typing Techniques Carbon-Oxygen Ligases / genetics, metabolism Conjugation, Genetic Copper / metabolism* Copper Sulfate / metabolism, pharmacology DNA, Bacterial / genetics Dietary Supplements* Drug Resistance, Bacterial* Electrophoresis, Gel, Pulsed-Field Enterococcus / drug effects, genetics*, isolation & purification Erythromycin / pharmacology Feces / microbiology Longitudinal Studies Microbial Sensitivity Tests Selection, Genetic Swine / microbiology |
| Chemical | |
Reg. No./Substance:
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0/Bacterial Proteins; 0/DNA, Bacterial; 0/TcrB protein, Enterococcus faecium; 114-07-8/Erythromycin; 7440-50-8/Copper; 7758-98-7/Copper Sulfate; EC 6.1.-/Carbon-Oxygen Ligases; EC 6.1.-/VanA ligase, Bacteria |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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