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Selection of primers for polymerase chain reaction.
MedLine Citation:
PMID:  21400260     Owner:  NLM     Status:  In-Data-Review    
One of the most important factors affecting the quality of polymerase chain reaction (PCR) is the choice of primers. Several rules should be observed when designing primers and, in general, the more DNA sequence information available, the better the chance of finding an "ideal" primer pair. Fortunately, not all primer selection criteria need be met in order to synthesize a clean, specific product, since the adjustment of PCR conditions (such as composition of the reaction mixture, temperature, and duration of PCR steps) may considerably improve the reaction specificity. Amplification of 200-400-bp DNA is the most efficient and, in these cases, one may design efficient primers simply by following a few simple rules described in this chapter. It is more difficult to choose primers for efficient amplification of longer DNA fragments, and use of an appropriate primer analysis software is worthwhile.
W Rychlik
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  15     ISSN:  1064-3745     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  1993  
Date Detail:
Created Date:  2011-03-14     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  31-40     Citation Subset:  IM    
National Biosciences, Plymouth, MN.
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