Document Detail

Selection of objective function in genome scale flux balance analysis for process feed development in antibiotic production.
MedLine Citation:
PMID:  18611443     Owner:  NLM     Status:  MEDLINE    
Using flux variability analysis of a genome scale metabolic network of Streptomyces coelicolor, a series of reactions were identified, from disparate pathways that could be combined into an actinorhodin-generating mini-network. Candidate process feed nutrients that might be expected to influence this network were used in process simulations and in silico predictions compared to experimental findings. Ranking potential process feeds by flux balance analysis optimisation, using either growth or antibiotic production as objective function, did not correlate with experimental actinorhodin yields in fed processes. However, the effect of the feeds on glucose assimilation rate (using glucose uptake as objective function) ranked them in the same order as in vivo antibiotic production efficiency, consistent with results of a robustness analysis of the effect of glucose assimilation on actinorhodin production.
Chiraphan Khannapho; Hongjuan Zhao; Bhushan K Bonde; Andrzej M Kierzek; Claudio A Avignone-Rossa; Michael E Bushell
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-06-17
Journal Detail:
Title:  Metabolic engineering     Volume:  10     ISSN:  1096-7184     ISO Abbreviation:  Metab. Eng.     Publication Date:  2008 Sep 
Date Detail:
Created Date:  2008-09-03     Completed Date:  2008-12-09     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9815657     Medline TA:  Metab Eng     Country:  Belgium    
Other Details:
Languages:  eng     Pagination:  227-33     Citation Subset:  IM    
Microbial Science Division, Faculty of Health and Medical Sciences, University of Surrey, Guildford, Surrey GU2 7XH, UK.
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MeSH Terms
Anthraquinones / metabolism
Anti-Bacterial Agents / biosynthesis*
Energy Metabolism / physiology*
Genome, Bacterial / physiology*
Glucose / metabolism*
Streptomyces coelicolor / genetics,  metabolism*
Grant Support
//Biotechnology and Biological Sciences Research Council
Reg. No./Substance:
0/Anthraquinones; 0/Anti-Bacterial Agents; 1397-77-9/actinorhodin; 50-99-7/Glucose

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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