| Selection of monoclonal antibody to hCG which localizes in human choriocarcinoma growing in the Syrian hamster cheek pouch. | |
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MedLine Citation:
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PMID: 6540755 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Six different monoclonal antibodies (MAbs) to human chorionic gonadotropin (hCG) were evaluated for radioimmunodetection in Golden Syrian Hamsters bearing a human choriocarcinoma in the cheek pouch (188-516 mIU of hCG/g). The affinity of three antibodies when determined were high and five of the antibodies were of IgG1 subclass. After i.v. injection of Staphylococcus Protein A (SPA) affinity purified radioiodinated antibodies, tissue distribution studies demonstrated that antibody 061 had higher target-to-nontarget ratios than the other five antibodies. The mean tumor:muscle ratio of 131I-MAb 061 was 27.5 in four animals, where the mean tumor:muscle ratio of four animals injected with 131I-labeled control monoclonal IgG1 Kappa from MOPC-21 murine myeloma (MOPC-21) was only 2.2. Sharp images of the cheek pouch tumor were obtained at five days following a tracer dose of 131I-061 MAb, but not with the same dose of a 131I-MOPC-21. |
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Authors:
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M B Khazaeli; L E Brown; G A Kabza; E W Sargent; W H Beierwaltes |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Hybridoma Volume: 3 ISSN: 0272-457X ISO Abbreviation: Hybridoma Publication Date: 1984 |
Date Detail:
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Created Date: 1984-10-24 Completed Date: 1984-10-24 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 8202424 Medline TA: Hybridoma Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 41-8 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antibodies, Monoclonal / immunology* Antibodies, Neoplasm / immunology Antibody Affinity Antibody Specificity Antigens, Neoplasm / immunology Choriocarcinoma / immunology* Chorionic Gonadotropin / immunology* Cricetinae Female Humans Mesocricetus Pregnancy Uterine Neoplasms / immunology* |
| Grant Support | |
ID/Acronym/Agency:
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T32 CA90915/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Antibodies, Monoclonal; 0/Antibodies, Neoplasm; 0/Antigens, Neoplasm; 0/Chorionic Gonadotropin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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