Document Detail

Selection of a chemically defined medium for culturing adult newt forelimb regenerates.
MedLine Citation:
PMID:  478566     Owner:  NLM     Status:  MEDLINE    
Three commercially available tissue-culture media were evaluated for their ability to support continued growth and differentiation of 14-day regenerates of adult newt forelimbs. Serums, embryo extracts, egg ultrafiltrates, and antimicrobial agents were avoided in this analysis. The hormones insulin and L-thyroxine were added to these chemically defined media to enhance continued cellular metabolism and growth. The optimum conditions appeared to be cultivated at 25 degrees C (pH 7.2 to 7.4) in media osmotically adjusted to conditions approximating amphibian blood values (i.e. 225 m0SM for 199, 244 m0SM for CMRL-1066, and 262 m0SM FOR L-15).
M E Conn; G E Dearlove; M H Dresden
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  In vitro     Volume:  15     ISSN:  0073-5655     ISO Abbreviation:  In Vitro     Publication Date:  1979 Jun 
Date Detail:
Created Date:  1979-11-28     Completed Date:  1979-11-28     Revised Date:  2009-11-11    
Medline Journal Info:
Nlm Unique ID:  0063733     Medline TA:  In Vitro     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  409-14     Citation Subset:  IM    
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MeSH Terms
Cell Differentiation
Culture Media*
Culture Techniques / methods*
Forelimb / cytology,  physiology*
Reg. No./Substance:
0/Culture Media

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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