Document Detail

Selection characterization on overlapping reading frame of multiple-protein-encoding P gene in Newcastle disease virus.
MedLine Citation:
PMID:  20079581     Owner:  NLM     Status:  MEDLINE    
The aim of this study was to characterize the molecular evolution of P and V protein genes of the Newcastle disease virus (NDV). The P gene sequences of 55 NDV isolates, representing different chronological and geographic origins, were obtained from GenBank. In this paper, the evolution of the specific regions of the NDV P gene, encoding the P and V proteins, was analyzed. The nucleotides from the shared P/V region encoded the co-amino terminus of the two proteins, while the P-V/V-P region was respectively encoded by the nucleotides within the P ORF or the V ORF in the common sequence (after the mRNA editing site). As well, the P-cut region exclusively encoded the P protein. Finally, the P-V and V-P regions were further broken down into P1 and P2 fragments with the corresponding V1 and V2 fragments. In the P gene, the P-cut portion corresponding to the C-terminal of the P protein was the most highly conserved, while the P-V region was the most variable. This was interpreted as a lower constraint for function in the common sequence than in the unique P sequence that is known to contain an important function. Interestingly, in the common P-V/V-P function, variability of V1 was compensated by a higher conservation of the corresponding P1, and conversely for the P2/V2, which suggested that the flexibility of one ORF with less function served the purpose of allowing positive selection in the other overlapping ORF that exhibited more function.
Jun-Wen Liang; Fu-Lin Tian; Zhou-Ran Lan; Bing Huang; Wen-Zhong Zhuang
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-12-28
Journal Detail:
Title:  Veterinary microbiology     Volume:  144     ISSN:  1873-2542     ISO Abbreviation:  Vet. Microbiol.     Publication Date:  2010 Aug 
Date Detail:
Created Date:  2010-08-16     Completed Date:  2010-12-21     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7705469     Medline TA:  Vet Microbiol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  257-63     Citation Subset:  IM    
Copyright Information:
Copyright 2009 Elsevier B.V. All rights reserved.
College of Life Science, Shandong Normal University, Wenhua East Road, Shandong Province, Jinan 250014, China.
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MeSH Terms
Base Sequence
Evolution, Molecular*
Genetic Variation
Newcastle disease virus / genetics*
Selection, Genetic*
Viral Proteins / genetics,  metabolism*
Reg. No./Substance:
0/Viral Proteins

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