Document Detail


Selection and characterization of colorectal cancer cell-specific peptides.
MedLine Citation:
PMID:  23338703     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
To develop a novel peptide probe for imaging detection of colorectal cancer, a 12-mer phage display library was used to select peptides that bind specifically to the human colorectal cancer cell line Caco-2. After four rounds of panning, four phage clones that bound specifically to the Caco-2 cells were selected. The phage clone SP-2 had a particularly high affinity and specificity for Caco-2 cells. This clone was identified using a series of methods. The peptide SP-2 that was displayed on phage SP-2 exhibited a high specificity to Caco-2 cells. Thus, the peptide SP-2 could be a candidate probe for the detection of colorectal cancer.
Authors:
Han Wang; Caixia Ma; Rui Li; Yonge Guo; Yakun He; Xiaojing Wang; Yiwen Chen; Yingchun Hou
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-22
Journal Detail:
Title:  Biotechnology letters     Volume:  -     ISSN:  1573-6776     ISO Abbreviation:  Biotechnol. Lett.     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-22     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8008051     Medline TA:  Biotechnol Lett     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Department of Cell Biology, College of Life Sciences, Shaanxi Normal University, 199 South Chang'an Road, Xi'an, 710062, People's Republic of China.
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