| Selection of aptamers against Ara h 1 protein for FO-SPR biosensing of peanut allergens in food matrices. | |
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MedLine Citation:
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PMID: 23318547 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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The rising prevalence to food allergies in the past two decades, together with the fact that the only existing therapy is avoidance of allergen-containing food next to the implementation of anti-allergic drugs, urges the need for improved performance of current assays to detect potential allergens in food products. Therein, the focus has been on aptamer-based biosensors in recent years. In this paper we report for the first time the selection of aptamers against one of the most important peanut allergens, Ara h 1. Several Ara h1 DNA aptamers were selected after eight selection rounds using capillary electrophoresis (CE)-SELEX. The selected aptamers specifically recognized Ara h 1 and did not significantly bind with other proteins, including another peanut allergen Ara h 2. The dissociation constant of a best performing aptamer was in the nanomolar range as determined independently by three different approaches, which are surface plasmon resonance, fluorescence anisotropy, and capillary electrophoresis (353±82nM, 419±63nM, and 450±60nM, respectively). Furthermore, the selected aptamer was used for bioassay development on a home-built fiber optic surface plasmon resonance (FO-SPR) biosensor platform for detecting Ara h 1 protein in both buffer and food matrix samples demonstrating its real potential for the development of novel, more accurate aptamer-based biosensors. In conclusion, the reported aptamer holds a great potential for the detection of Ara h 1 in both the medical field and the food sector due to its high affinity and specificity for the target protein. |
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Authors:
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Dinh T Tran; Karel Knez; Kris P Janssen; Jeroen Pollet; Dragana Spasic; Jeroen Lammertyn |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-12-20 |
Journal Detail:
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Title: Biosensors & bioelectronics Volume: 43C ISSN: 1873-4235 ISO Abbreviation: Biosens Bioelectron Publication Date: 2012 Dec |
Date Detail:
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Created Date: 2013-1-15 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9001289 Medline TA: Biosens Bioelectron Country: - |
Other Details:
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Languages: ENG Pagination: 245-251 Citation Subset: - |
Copyright Information:
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Copyright © 2012 Elsevier B.V. All rights reserved. |
Affiliation:
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BIOSYST-MeBioS, KU Leuven-University of Leuven, Willem de Croylaan 42, B-3001 Leuven, Belgium. Electronic address: ttdinh@hua.edu.vn. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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