Document Detail


Selection of aptamers against Ara h 1 protein for FO-SPR biosensing of peanut allergens in food matrices.
MedLine Citation:
PMID:  23318547     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
The rising prevalence to food allergies in the past two decades, together with the fact that the only existing therapy is avoidance of allergen-containing food next to the implementation of anti-allergic drugs, urges the need for improved performance of current assays to detect potential allergens in food products. Therein, the focus has been on aptamer-based biosensors in recent years. In this paper we report for the first time the selection of aptamers against one of the most important peanut allergens, Ara h 1. Several Ara h1 DNA aptamers were selected after eight selection rounds using capillary electrophoresis (CE)-SELEX. The selected aptamers specifically recognized Ara h 1 and did not significantly bind with other proteins, including another peanut allergen Ara h 2. The dissociation constant of a best performing aptamer was in the nanomolar range as determined independently by three different approaches, which are surface plasmon resonance, fluorescence anisotropy, and capillary electrophoresis (353±82nM, 419±63nM, and 450±60nM, respectively). Furthermore, the selected aptamer was used for bioassay development on a home-built fiber optic surface plasmon resonance (FO-SPR) biosensor platform for detecting Ara h 1 protein in both buffer and food matrix samples demonstrating its real potential for the development of novel, more accurate aptamer-based biosensors. In conclusion, the reported aptamer holds a great potential for the detection of Ara h 1 in both the medical field and the food sector due to its high affinity and specificity for the target protein.
Authors:
Dinh T Tran; Karel Knez; Kris P Janssen; Jeroen Pollet; Dragana Spasic; Jeroen Lammertyn
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-12-20
Journal Detail:
Title:  Biosensors & bioelectronics     Volume:  43C     ISSN:  1873-4235     ISO Abbreviation:  Biosens Bioelectron     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2013-1-15     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9001289     Medline TA:  Biosens Bioelectron     Country:  -    
Other Details:
Languages:  ENG     Pagination:  245-251     Citation Subset:  -    
Copyright Information:
Copyright © 2012 Elsevier B.V. All rights reserved.
Affiliation:
BIOSYST-MeBioS, KU Leuven-University of Leuven, Willem de Croylaan 42, B-3001 Leuven, Belgium. Electronic address: ttdinh@hua.edu.vn.
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