| Selection of anionic dopant for quantifying desialylation reactions with MALDI-FTMS. | |
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MedLine Citation:
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PMID: 10763235 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Alkylsulfonates are examined as anion dopants for the matrix-assisted laser desorption/ionization-mass spectrometry (MALDI-MS) of neutral oligosaccharides. The anion dopants allow neutral oligosaccharides to be examined in the same mixture as acidic oligosaccharides. The alkylsulfonate dopants interact strongly with the oligosaccharide to produce a quasimolecular ion composed of the oligosaccharides and the deprotonated alkylsulfonates. Compounds as small as disaccharides to large branched oligosaccharides can all be examined. The new series of alkylsulfonates works as well as HSO4- in terms of signal-to-noise ratio, sensitivity, and ease of preparation in the negative ion mode. The alkylsulfonates are used to construct a calibration curve for obtaining the relative amounts of varying neutral and acidic oligosaccharide mixtures. With the anion dopants, the MALDI-FTMS signals are shown to have a linear relationship with the molar ratios of the neutral and sialylated oligosaccharides. The method is also used to monitor the product of a desialylation reaction by acid hydrolysis. |
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Authors:
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A W Wong; H Wang; C B Lebrilla |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Analytical chemistry Volume: 72 ISSN: 0003-2700 ISO Abbreviation: Anal. Chem. Publication Date: 2000 Apr |
Date Detail:
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Created Date: 2000-04-27 Completed Date: 2000-04-27 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0370536 Medline TA: Anal Chem Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1419-25 Citation Subset: IM |
Affiliation:
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Department of Chemistry, University of California, Davis 95616, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Anions Fourier Analysis N-Acetylneuraminic Acid / chemistry* Oligosaccharides / chemistry Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods* |
| Grant Support | |
ID/Acronym/Agency:
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GM4907701/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Anions; 0/Oligosaccharides; 131-48-6/N-Acetylneuraminic Acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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