Document Detail


Selection of anionic dopant for quantifying desialylation reactions with MALDI-FTMS.
MedLine Citation:
PMID:  10763235     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Alkylsulfonates are examined as anion dopants for the matrix-assisted laser desorption/ionization-mass spectrometry (MALDI-MS) of neutral oligosaccharides. The anion dopants allow neutral oligosaccharides to be examined in the same mixture as acidic oligosaccharides. The alkylsulfonate dopants interact strongly with the oligosaccharide to produce a quasimolecular ion composed of the oligosaccharides and the deprotonated alkylsulfonates. Compounds as small as disaccharides to large branched oligosaccharides can all be examined. The new series of alkylsulfonates works as well as HSO4- in terms of signal-to-noise ratio, sensitivity, and ease of preparation in the negative ion mode. The alkylsulfonates are used to construct a calibration curve for obtaining the relative amounts of varying neutral and acidic oligosaccharide mixtures. With the anion dopants, the MALDI-FTMS signals are shown to have a linear relationship with the molar ratios of the neutral and sialylated oligosaccharides. The method is also used to monitor the product of a desialylation reaction by acid hydrolysis.
Authors:
A W Wong; H Wang; C B Lebrilla
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Analytical chemistry     Volume:  72     ISSN:  0003-2700     ISO Abbreviation:  Anal. Chem.     Publication Date:  2000 Apr 
Date Detail:
Created Date:  2000-04-27     Completed Date:  2000-04-27     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0370536     Medline TA:  Anal Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1419-25     Citation Subset:  IM    
Affiliation:
Department of Chemistry, University of California, Davis 95616, USA.
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MeSH Terms
Descriptor/Qualifier:
Anions
Fourier Analysis
N-Acetylneuraminic Acid / chemistry*
Oligosaccharides / chemistry
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*
Grant Support
ID/Acronym/Agency:
GM4907701/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Anions; 0/Oligosaccharides; 131-48-6/N-Acetylneuraminic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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