Document Detail


Selection of targeted mutants from a library of randomly mutagenized ES cells.
MedLine Citation:
PMID:  21080287     Owner:  NLM     Status:  In-Process    
Abstract/OtherAbstract:
A method for random relatively unbiased mutagenesis of ES cells with a mutagenic retroviral vector is described. An orderly assembly of mutant ES cells in multi-well plates is generated. 3D pooling of the wells of the assembly allows quick PCR search for insertions in genes of interest. Mutant ES cell clones are then isolated from the positive wells and used to produce mutant animals using conventional techniques.
Authors:
Kyoji Horie; George Gaitanaris; Alexander Gragerov
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  693     ISSN:  1940-6029     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2011  
Date Detail:
Created Date:  2010-11-16     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  283-94     Citation Subset:  IM    
Affiliation:
Omeros Corporation, Seattle, WA, USA.
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