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Selection of Rattus norvegicus oocytes for in vitro maturation by brilliant cresyl blue staining.
MedLine Citation:
PMID:  21791168     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
SummaryThe objective of this work was to evaluate the rate of meiosis resumption and nuclear maturation of rat (Rattus norvegicus) oocytes selected for in vitro maturation (IVM) after staining of cumulus-oocyte complexes (COCs) with blue cresyl brilliant (BCB) using different protocols: exposure for 30, 60 or 90 min at 26 μM BCB (Experiment 1), and exposure for 60 min at 13, 20 or 26 μM BCB (Experiment 2). In Experiment 1, the selection of oocytes exposed to BCB for 60 min was found to be the most suitable, as meiosis resumption rates in the BCB+ group (n = 35/61; 57.37%) were the closest to the observed in the control (not exposed) group (n = 70/90; 77.77%) and statistically higher than the values observed for the BCB- group (n = 3/41; 7.32%). Additionally, the more effective evaluation of diagnostic tests (sensitivity and negative predictive value 100%) was observed in COCs exposed for 60 min. In Experiment 2, the 13 μM BCB+ group presented rates of meiosis resumption (n = 57/72; 72.22%) similar to the control group (n = 87/105; 82.86%) and higher than other concentration groups. However, this results of the analysis between BCB- oocytes was also higher in the 13 μM BCB group (n = 28/91; 30.78%) when compared with BCB- COCs exposed to 20 μM (n = 3/62; 4.84%) or 26 μM (n = 3/61; 4.92%) BCB. The nuclear maturation rate in the 13 μM BCB group was similar between BCB+ or BCB- oocytes. The 20 μM BCB group had a lower rate of nuclear maturation of BCB- oocytes than other groups. Thus, our best results in the selection of Rattus norvegicus oocytes by staining with BCB were obtained using the concentration of 13 μM and 20 μM, and an incubation period of 60 min.
Authors:
Diego Duarte Alcoba; Bianca Letícia da Rosa Braga; Nathallie Louise Sandi-Monroy; Letícia Auler Proença; Rui Fernando Felix Lopes; Alexandre Tavares Duarte de Oliveira
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-7-27
Journal Detail:
Title:  Zygote (Cambridge, England)     Volume:  -     ISSN:  1469-8730     ISO Abbreviation:  -     Publication Date:  2011 Jul 
Date Detail:
Created Date:  2011-7-27     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9309124     Medline TA:  Zygote     Country:  -    
Other Details:
Languages:  ENG     Pagination:  1-8     Citation Subset:  -    
Affiliation:
Laboratório de Biologia Celular-Universidade Federal de Ciências da Saúde de Porto Alegre (UFCSPA)-Porto Alegre, Brazil.
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