Document Detail

Selected neurotrophins, neuropeptides, and cytokines: developmental trajectory and concentrations in neonatal blood of children with autism or Down syndrome.
MedLine Citation:
PMID:  16289943     Owner:  NLM     Status:  MEDLINE    
Using a double-antibody immunoaffinity assay (Luminex) and ELISA technology, we measured concentrations of certain neurotrophins, neuropeptides, and cytokines in pooled samples (one to three subjects per sample) eluted from archived neonatal blood of children with later-diagnosed autism, Down syndrome, very preterm birth, or term control infants. We also measured analytes in blood from healthy adult controls. Case or control status for infant subjects was ascertained by retrospective review of service agency medical records. We observed inhibitory substances in eluates from archived bloodspots, especially marked for measurement of BDNF. Concentrations in control subjects differed by age: BDNF rose markedly with age, while NT-3 and NT-4/5 concentrations were lower in adults than in newborn infants. IL-8 concentrations were higher in newborn infants, preterm and term, than in adults. Considered by diagnostic group, total protein was higher in Down syndrome than in either autism or control subjects. In infants with Down syndrome, concentrations of IL-8 levels were higher than in controls, whether or not corrected for total protein; NT-3 and CGRP were lower and VIP higher. In samples from autistic subjects, NT-3 levels were significantly lower than controls and an increase in VIP approached statistical significance. Concentrations of NT-4/5 and CGRP were correlated in infants with autism but not in Down syndrome or controls. Some of these results differ from earlier findings using a single-antibody recycling immunoaffinity chromatography (RIC) system. We discuss interrelationships of VIP, NT-3 and IL-8 and their potential relevance to features of the neuropathology of autism or Down syndrome.
Phillip G Nelson; Thea Kuddo; Eun Young Song; James M Dambrosia; Shawn Kohler; Gowri Satyanarayana; Cassandra Vandunk; Judith K Grether; Karin B Nelson
Related Documents :
6547633 - Northern infant syndrome: a deficiency state?
17956373 - Sleep practices and environment and the risk of sudden infant death syndrome in turkey.
3484463 - Absence of late effects on survival and developmental abilities of pachytene oocytes x-...
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Intramural; Research Support, Non-U.S. Gov't     Date:  2005-11-14
Journal Detail:
Title:  International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience     Volume:  24     ISSN:  0736-5748     ISO Abbreviation:  Int. J. Dev. Neurosci.     Publication Date:  2006 Feb 
Date Detail:
Created Date:  2006-02-28     Completed Date:  2006-06-26     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8401784     Medline TA:  Int J Dev Neurosci     Country:  England    
Other Details:
Languages:  eng     Pagination:  73-80     Citation Subset:  IM    
National Institute of Child Health and Development, Building 31, Room 2A25, Bethesda, MD 20892-2426, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Age Factors
Autistic Disorder / blood*
Brain-Derived Neurotrophic Factor / blood
Calcitonin Gene-Related Peptide / blood
Down Syndrome / blood*
Enzyme-Linked Immunosorbent Assay
Gestational Age
Infant, Newborn
Interleukin-8 / blood*
Nerve Growth Factors / blood
Neurotrophin 3 / blood*
Retrospective Studies
Vasoactive Intestinal Peptide / blood*
Reg. No./Substance:
0/Brain-Derived Neurotrophic Factor; 0/Interleukin-8; 0/Nerve Growth Factors; 0/Neurotrophin 3; 143551-63-7/neurotrophin 4; 37221-79-7/Vasoactive Intestinal Peptide; 83652-28-2/Calcitonin Gene-Related Peptide

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Huntingtin is localized in the nucleus during preimplanatation embryo development in mice.
Next Document:  Calretinin-immunoreactive unipolar brush cells in the developing human cerebellum.