Document Detail


Seizures during pregnancy modify the development of hippocampal interneurons of the offspring.
MedLine Citation:
PMID:  20708978     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We investigated the effect of epileptic seizures during pregnancy on hippocampal expression of calcium-binding proteins in the offspring. Female Wistar rats were submitted to the pilocarpine model and mated during the chronic period. Seizure frequency was monitored over the entire pregnancy. Pups were perfused at postnatal days 6 and 13, and the brains processed for Nissl staining and immunohistochemistry for NeuN, calbindin, calretinin, and parvalbumin. Number of stained cells in the hippocampus was estimated through stereological methods. Our results showed a decrease in epileptic seizure frequency during pregnancy. No differences were observed in NeuN-positive, CR-positive cells, and Nissl-stained hippocampal neurons between the groups. However, there was a significant decrease in calbindin-positive cells (P=0.005) and a significant increase in parvalbumin-positive cells (P=0.02) in the experimental group when compared with the control group. These results suggest that seizures during pregnancy affect the development of specific hippocampal interneurons of the offspring.
Authors:
Tiago Gurgel do Vale; Alexandre Valotta da Silva; Daiana Correia Lima; Eliângela de Lima; Laila Brito Torres; Ana Carolina Cossa; Elaine Menezes de Oliveira; Francisco Romero Cabral; Esper Abrão Cavalheiro; Maria da Graça Naffah-Mazzacoratti; Débora Amado
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-08-14
Journal Detail:
Title:  Epilepsy & behavior : E&B     Volume:  19     ISSN:  1525-5069     ISO Abbreviation:  Epilepsy Behav     Publication Date:  2010 Sep 
Date Detail:
Created Date:  2010-09-20     Completed Date:  2011-01-12     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100892858     Medline TA:  Epilepsy Behav     Country:  United States    
Other Details:
Languages:  eng     Pagination:  20-5     Citation Subset:  IM    
Copyright Information:
Copyright © 2010 Elsevier Inc. All rights reserved.
Affiliation:
Disciplina de Neurologia Experimental, Universidade Federal de São Paulo, São Paulo, Brasil.
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MeSH Terms
Descriptor/Qualifier:
Age Factors
Animals
Animals, Newborn
Calcium-Binding Proteins / metabolism
Cell Count / methods
Disease Models, Animal
Female
Hippocampus / growth & development*,  pathology*
Interneurons / pathology*
Male
Phosphopyruvate Hydratase / metabolism
Pilocarpine
Pregnancy
Prenatal Exposure Delayed Effects / pathology*,  physiopathology
Rats
Rats, Wistar
Seizures / chemically induced,  pathology*
Chemical
Reg. No./Substance:
0/Calcium-Binding Proteins; 92-13-7/Pilocarpine; EC 4.2.1.11/Phosphopyruvate Hydratase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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